CENTRIFUGAL FAST CHROMATOGRAPH

我们的目标是彻底改变高性能液体 使用新原理和新分析系统的色谱法 其允许对许多样品进行多并行分析， 分辨率、自动数据采集和数据分析， 显示. 高分辨率所需的高压是 通过离心力获得，该离心力也将样品从 将圆盘转移到色谱柱上。 的梯度 分段装置将一个洗脱流切割成小部分 其被同等地引入到所有柱中。 洗脱密度 梯度，当与离心力相结合时， 样品区和所有分离区就位，以及 垂直于柱的半径。 密度 梯度也会在流动过程中保持紧密区域 cell. 提供了两个流分段设备， 与适当阀门配合，流入内侧或外侧 方向穿过柱子。 使用流动池进行外侧洗脱 向心的列是用梯度递减 密度，而使用内侧方向的洗脱 密度逐渐增加的梯度。 由于该系统没有泵， 平行运行多个柱，并使用一个光学系统， 微处理器的数据采集和分析，并提供 我们相信，当这个系统以很高的速度分析时， 完善，取代了大部分的HPLC系统， 目前使用。

Our objective is to revolutionize high performance liquid chromatography using new principles and a new analytical system which allows multiple-parallel analysis of many samples with high resolution, automatic data acquisition, and data analysis and display. The high pressures required for high resolution are obtained by centrifugal force, which also moves samples from a transfer disc to the chromatographic columns. A gradient segmentation device cuts one elution stream into small fractions which are introduced equally into all columns. An eluting density gradient, when combined with centrifugal force, maintains the sample zone, and all separated zones in position, and perpendicular to a radius through the column. The density gradient also keeps tight zones as they move through the flow cell. Two stream segmentation devices are provided to allow, in concert with suitable valving, flow in either inboard or outboard direction through the column. Outboard elution with the flow cell centripetal to the column is used with gradients of decreasing density, while elution in an inboard direction is used with gradients of increasing density. Since the system has no pumps, runs many columns in parallel, and uses one optical system and microprocessor for data acquisition and analysis, and provides analyses at a high rate, we believe that this system will, when perfected, supplant a large fraction of the HPLC systems in present use.