CAMERA SYSTEM FOR TWO-DIMENSIONAL ELECTROPHORESIS GELS

二维电泳凝胶摄像系统

• 批准号: 3497820
• 负责人: NORMAN G ANDERSON
• 金额: $ 5万
• 依托单位: LARGE SCALE BIOLOGY CORPORATION
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-02-21 至 1986-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3497820
• 关键词: densitometry; gel electrophoresis; image enhancement; image processing; photography

While a variety of scanners and camera systems have been described for acquiring two-dimensional electrophoretic gel patterns for display, analysis, intercomparison, and reproduction, none solves all of the problems of image acquisition and image identification in a satisfactory manner. This proposal is for the development of a 70-mm precision pin-registered camera system for photographing two-dimensional electrophoretic patterns. A second optical path includes in the negative a CRT-generated identification and data display area. A unique design reduces lens flare to a minimum. Using a three color filter system, color separation negatives may also be made in sequence for subsequent color reproduction. The camera system is also a 70-mm printer which exposes positive film to the negative film. The positives may be viewed and intercompared on a merging comparator, or digitized for computerized image analysis. Incorporated into the printing system is a unique technique for removing dense background areas such as may exist in silver stained gels. The system is flexible, and retains the original image for study or reproduction. One centralized digitizing scanner can serve numerous remote 70 mm gel cameras. We believe this system is the one which will come into widest use.

而各种扫描器和摄像系统已被描述