FAST CLINICAL IMMUNOVIROLOGY

目标是开发新的快速检测方法， 分离和鉴定可能出现的新病毒，已知病毒粒子， 病毒抗原和已知致病病毒的抗体。这些方法可用于 取决于独特的离心机技术组合，以及 两个个体的带状密度和沉积系数 病毒颗粒的种类以及荧光和非荧光的 乳胶颗粒。方法包括(按递增的顺序 仪器复杂性)密度分离的荧光 免疫聚集体，速率分离的荧光免疫聚集体， 等密度病毒检测和二维离心式病毒 与世隔绝。通过这些方法，人们提出了新的病毒可以 快速分离和表征，可以开发出测试，将产生 精确的病毒滴度允许对培养病毒的方法进行评估 即使获得的产量非常低，也可以开发出快速的方法 基于病毒粒子检测的病毒疾病诊断，抗原 检测，或抗体检测。第一阶段是原则证明阶段。 第二阶段是原型开发和初步应用。

The objectives are to develop new and rapid methods for detecting, isolating and characterizing new viruses which may appear, known virions, viral antigens, and antibodies to known pathogenic viruses. The methods to be explored depend on unique combinations of centrifugal techniques, and the banding densities and sedimentation coefficients of both individual species of virus particles and of both fluorescent and non-fluorescent latex particles. The methods include (in order of increased instrumentational complexity) density-separated fluorescent immunoaggregates, rate-separated fluorescent immunoaggregates, isopycnometric virus detection, and two-dimensional centrifugal virus isolation. With these methods it is proposed that new viruses can be rapidly isolated and characterized, tests can be developed which will yield precise virus titers allowing methods for growing the virus to be evaluated even when very poor yields are obtained, and rapid methods can be developed for diagnosis of virus diseases based on virion detection, antigen detection, or antibody detection. Phase I is for proof of principle. Phase II is for prototype development and initial applications.