CANCER PROTEIN DATA BASE

高分辨率二维电泳允许大部分的 预计将有30，000到50，000种人类蛋白质被图谱识别出来， 位置和数量。 正常细胞和癌细胞已经被绘制出来 在定向研究中发现了许多差异。为了给这些 结果，并系统地利用它们，一个大的数据库（癌症 蛋白质数据库），包括所有可用的和所有可获得的数据， 点是必需的，数据库直接连接到CRT地图显示。 数据库将包括（对于每种蛋白质）细胞内位置、类型 在其中发现的细胞，名称，功能，疾病相关性， 生物物理学数据，文献引用，共调节组的成员， 发生的分化阶段，和染色体的位置， 编码基因 需要探讨的论点是，细胞是高度 整合系统，以蛋白质组和蛋白质组的方式来理解， 涉及它们的调节回路， 癌细胞不是随机的，而是反映了正常调节的改变。 和微分函数。 第一阶段的提案是为了 继续开发TYCHO和KEPLER数据库系统， 专门针对癌症研究，在一个私人的设置。

High resolution two dimensional electrophoresis allows a large fraction of the estimated 30,000 to 50,000 human proteins to be identified by map position and to be quantitated. Normal and cancer cells have been mapped in orienting studies and many differences found. To give meaning to these results, and to systematically exploit them, a large data base (the Cancer Protein Data Base) including all available and all obtainable data on each spot is required, with the data base directly linked to CRT map displays. The data base will include (for each protein) intracellular location, types of cells in which found, names, functions, disease correlations, biophysical data, literature citations, membership in coregulated sets, differentiational stage of occurrence, and chromosomal location of the coding gene. The theses to be explored are that cells are highly integrated systems to be understood in terms of protein sets and the regulational circuits involving them, and that compositional changes in cancer cells are not random, but reflect alterations in normal regulational and differentiational functions. This Phase I proposal is for the continued development of the TYCHO and KEPLER data base systems, specifically directed to cancer studies, in a private setting.