DETECTING AND QUANTITATING UNCULTURABLE HUMAN PATHOGENS

检测和定量不可培养的人类病原体

• 批准号: 2887558
• 负责人: NORMAN G ANDERSON
• 金额: $ 37.5万
• 依托单位: LARGE SCALE BIOLOGY CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887558
• 关键词: biotechnology; communicable disease diagnosis; density gradient ultracentrifugation; diagnosis design /evaluation; fluorescent dye /probe; lasers; method development; serology /serodiagnosis; virus characteristic; virus load

DESCRIPTION: (Adapted from the applicant's abstract) This proposal is to develop a rapid method for separating, identifying and counting small numbers of viral pathogens from tissues, blood, urine, water supplies, and natural waters, with emphasis on agents currently unculturable. It is based on the observation that in nature there are few particles of viral dimensions that have a narrow range of both sedimentation rates and isopycnic banding densities, which are not viruses. In Phase I, a prototype centrifugal concentration and microbanding system was constructed and tested which employs a density gradient, density standards, laser illumination and staining with DNA-binding fluorescent dyes. Virus particles from 4 mL samples have been concentrated to a cubic micron, yielding PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人摘要）本提案旨在 开发一种快速分离、识别和计数小 来自组织、血液、尿液、水源的病毒病原体数量，以及 自然沃茨，重点是目前不可培养的媒介。 是 基于自然界中很少有病毒颗粒的观察， 尺寸，有一个狭窄的范围内的沉积速率和 等密度带密度，这不是病毒。 在第一阶段， 原型离心浓缩和微带系统是 构建和测试，其采用密度梯度，密度 标准品、激光照射和DNA结合荧光染色 染料。 将来自4 mL样品的病毒颗粒浓缩至立方 微米，屈服 拟议商业应用：不可用