IMMUNODOMINANT TARGETS OF CMI TO CRYPTOSPORIDIUM

CMI 对隐孢子虫的免疫显性靶点

• 批准号: 2672387
• 负责人: JAN R MEAD
• 金额: $ 17.47万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2672387
• 关键词: Cryptosporidium; SCID mouse; T lymphocyte; cellular immunity; clone cells; cryptosporidiosis; genetic library; interleukin 2; interleukin 4; interleukin 5; lymphocyte proliferation; microorganism immunology; passive immunization; protozoal antigen; recombinant proteins; tissue /cell culture; western blottings

DESCRIPTION: (Adapted From Applicant's Abstract). This second resubmission entitled "Immunodominant Targets of CMI to Cryptosporidium" is to identify important cryptosporidial antigens in immune response and recovery. The applicant proposes to identify and isolate recombinant C. parvum antigens from a cDNA library using polyclonal and monoclonal antibodies generated against cryptosporidial proteins, to evaluate purified recombinant proteins for their immunogenicity using a T cell proliferation assay as either gel purified protein or a nitrocellulose- bound protein (T cell immunoblot assay) in order to determine whether these antigens elicit T cell responses and finally to isolate T cell clones that elicit strong responses to these antigens and then engraft them to infected SCID mice to determine whether these can reduce parasite load or induce recovery.

描述：（改编自申请人摘要）。这第二个