TRANSCRIPTION--INDUCED MUTATIONS

描述：存储在DNA中的遗传信息受到保护，不受攻击 由于这种分子的双链性质，许多化学物质。这 保护减少突变并确保更大的基因稳定性 材料。然而，复制遗传物质需要临时的 DNA链的分离使它们暴露在化学危险中。这个 最近，研究人员使用遗传方法证明了 转录在非转录链上造成C到T突变 大肠埃希菌基因。数据表明，这种情况的发生是因为 非转录链中胞嘧啶的水解性脱氨基 再高一点。他提出了一些实验，这些实验将建立 这一现象，表明其他基因也是如此，并测试了 某些化学诱变剂可能也有类似的链 偏见。为了实现这一点，他将复制特定于链的突变 并在体外测试了几个关于这些突变的预测 目前对转录在大肠杆菌中的理解。由此产生的结果是 工作应该为诱变剂的作用创造一个新的视角，并提供一种 对成绩单延长的过程有更多的了解。

DESCRIPTION: The genetic information stored in DNA is protected from attack of many chemicals by the double-stranded nature of this molecule. This protection reduces mutations and assures greater stability for the genetic material. However, copying of the genetic material requires temporary separation of the DNA strands exposing them to chemical hazards. The investigator recently used genetic method to show that the process of transcription creates C to T mutations in the non-transcribed strand of an Escherichia coli gene. The data suggested that this occurs because the rate of hydrolytic deamination of cytosines in the non-transcribed strand is higher. He proposes experiments tha will establish the biochemical basis of this phenomenon, show that it is true of other genes, and test the possibility that certain chemical mutagens may also have a similar strand bias. To accomplish this, he will reproduce the strand-specific mutations in vitro and test several predictions regarding thes mutations based on current understanding of transcription in E. coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation.