TRANSCRIPTION--INDUCED MUTATIONS

转录——诱导突变

• 批准号: 2561202
• 负责人: ASHOK S BHAGWAT
• 金额: $ 20.23万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2561202
• 关键词: DNA directed RNA polymerase; Escherichia coli; alkylating agents; bacterial genetics; enzyme activity; gene mutation; genetic transcription; microorganism culture; mutagen testing; mutagens; nitrous acid; sulfites

DESCRIPTION: The genetic information stored in DNA is protected from attack of many chemicals by the double-stranded nature of this molecule. This protection reduces mutations and assures greater stability for the genetic material. However, copying of the genetic material requires temporary separation of the DNA strands exposing them to chemical hazards. The investigator recently used genetic method to show that the process of transcription creates C to T mutations in the non-transcribed strand of an Escherichia coli gene. The data suggested that this occurs because the rate of hydrolytic deamination of cytosines in the non-transcribed strand is higher. He proposes experiments tha will establish the biochemical basis of this phenomenon, show that it is true of other genes, and test the possibility that certain chemical mutagens may also have a similar strand bias. To accomplish this, he will reproduce the strand-specific mutations in vitro and test several predictions regarding thes mutations based on current understanding of transcription in E. coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation.

描述：DNA 中存储的遗传信息受到保护，免受攻击 通过该分子的双链性质，许多化学物质。 这 保护减少突变并确保遗传的更大稳定性 材料。 然而，遗传物质的复制需要暂时的 DNA 链的分离使它们暴露于化学危害。 这 研究人员最近使用遗传方法证明了这一过程 转录在非转录链中产生 C 到 T 的突变 大肠杆菌基因。 数据表明，发生这种情况的原因是 非转录链中胞嘧啶的水解脱氨作用是 更高。 他提出了一些实验来建立生物化学基础 这种现象，表明其他基因也是如此，并测试 某些化学诱变剂也可能具有类似链的可能性 偏见。 为了实现这一目标，他将复制链特异性突变 体外并测试基于这些突变的几种预测 目前对大肠杆菌转录的了解。 由此得出的结果 工作应该为诱变剂的作用创造一个新的视角，并提供 更好地了解转录本延伸的过程。