TRANSCRIPTION--INDUCED MUTATIONS

转录——诱导突变

• 批准号: 6180490
• 负责人: ASHOK S BHAGWAT
• 金额: $ 20.87万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180490
• 关键词: DNA directed RNA polymerase; Escherichia coli; alkylating agents; bacterial genetics; enzyme activity; gene mutation; genetic transcription; microorganism culture; mutagen testing; mutagens; nitrous acid; sulfites

DESCRIPTION: The genetic information stored in DNA is protected from attack of many chemicals by the double-stranded nature of this molecule. This protection reduces mutations and assures greater stability for the genetic material. However, copying of the genetic material requires temporary separation of the DNA strands exposing them to chemical hazards. The investigator recently used genetic method to show that the process of transcription creates C to T mutations in the non-transcribed strand of an Escherichia coli gene. The data suggested that this occurs because the rate of hydrolytic deamination of cytosines in the non-transcribed strand is higher. He proposes experiments tha will establish the biochemical basis of this phenomenon, show that it is true of other genes, and test the possibility that certain chemical mutagens may also have a similar strand bias. To accomplish this, he will reproduce the strand-specific mutations in vitro and test several predictions regarding thes mutations based on current understanding of transcription in E. coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation.

描述：存储在DNA中的遗传信息受到保护，免受攻击 许多化学物质的双链性质。 这 保护减少了突变，并确保了基因的更大稳定性。 材料 然而，遗传物质的复制需要暂时的 DNA链的分离使它们暴露在化学危害中。 的 研究人员最近使用遗传学方法表明， 转录在非转录链中产生C至T突变， 大肠杆菌基因。 数据表明，发生这种情况是因为 非转录链中胞嘧啶的水解脱氨基作用是 高 他提出了一些实验，这些实验将建立生物化学基础， 这一现象，表明这是真实的其他基因，并测试 某些化学诱变剂也可能具有类似的链， bias. 为了达到这个目的，他将复制链特异性突变 在体外，并测试几个预测的基础上，这些突变 目前对E.杆菌 由此产生的结果 这项工作应该为诱变剂的作用创造一个新的视角， 更好地理解转录延长的过程。