TRANSCRIPTION--INDUCED MUTATIONS

转录——诱导突变

• 批准号: 6386832
• 负责人: ASHOK S BHAGWAT
• 金额: $ 21.34万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386832
• 关键词: DNA directed RNA polymerase; Escherichia coli; alkylating agents; bacterial genetics; enzyme activity; gene mutation; genetic transcription; microorganism culture; mutagen testing; mutagens; nitrous acid; sulfites

DESCRIPTION: The genetic information stored in DNA is protected from attack of many chemicals by the double-stranded nature of this molecule. This protection reduces mutations and assures greater stability for the genetic material. However, copying of the genetic material requires temporary separation of the DNA strands exposing them to chemical hazards. The investigator recently used genetic method to show that the process of transcription creates C to T mutations in the non-transcribed strand of an Escherichia coli gene. The data suggested that this occurs because the rate of hydrolytic deamination of cytosines in the non-transcribed strand is higher. He proposes experiments tha will establish the biochemical basis of this phenomenon, show that it is true of other genes, and test the possibility that certain chemical mutagens may also have a similar strand bias. To accomplish this, he will reproduce the strand-specific mutations in vitro and test several predictions regarding thes mutations based on current understanding of transcription in E. coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation.

描述：DNA中存储的遗传信息免受攻击 该分子的双链性质的许多化学物质。 这 保护减少突变并确保遗传的稳定性更大 材料。 但是，遗传材料的复制需要暂时 DNA链的分离将它们暴露于化学危险中。 这 研究者最近使用遗传学方法表明了 转录在非转录链中创建C到T突变 大肠杆菌基因。 数据表明，这是因为率 在非转录链中的细胞穿水解脱氨酸的IS 更高。 他提出实验将建立生化基础 这种现象表明，其他基因是正确的，并测试了 某些化学诱变剂的可能性也可能具有相似的链 偏见。 为此，他将重现特定于链的突变 体外和测试基于这些突变的几个预测 当前对大肠杆菌转录的理解。 从中的结果 工作应对诱变者的作用创造新的观点，并提供 对成绩单伸长过程的更多了解。