ENHANCEMENT OF FACULTY RESEARCH CAPABILITY

加强教师研究能力

• 批准号: 2910010
• 负责人: PURUSHOTTAM G KALE
• 金额: $ 2.63万
• 依托单位: ALABAMA AGRI AND MECH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2910010
• 关键词: Drosophilidae; developmental genetics; gene mutation; genetic mapping; genetic transcription; molecular cloning; radiation genetics; southern blotting

DESCRIPTION: This MORE faculty development award is to provide Dr. Kale with training in molecular biology. Dr. Kale will spend five consecutive summers investigating the Drosophila ted gene, under the guidance and in the lab of Dr. Cavener. This gene function is needed for the adult fly to emerge from the pupal case. The approximate chromosomal position of ted is known. Genomic clones of this chromosomal region are available. To clone ted, the gene must be localized more precisely within this region. Dr. Kale will begin this by inducing ted mutations with X-rays, and then mapping the breakpoints of these lesions via Southern blotting. Once the position of ted gene has been narrowed down, Dr. Kale will identify potential ted transcription units, isolate cDNA clones for those RNAs, and sequence and map the ted gene. Finally, to prove that he has indeed cloned ted, Dr. Kale will insert ted genomic DNA into a germline transformation vector and determine whether the DNA can rescue ted mutations.

描述：这个更多的教师发展奖是提供博士。 接受过分子生物学训练 凯尔医生将连续五年 萨默斯研究果蝇ted基因，在指导下， 卡文纳博士的实验室 这种基因的功能是成年果蝇 从蛹中出来 ted的大致染色体位置是 知道的 该染色体区域的基因组克隆是可用的。 克隆 ted，基因必须更精确地定位在这个区域内。 凯尔医生 将开始通过X射线诱导ted突变，然后绘制 通过Southern印迹分析这些病变的断点。 一旦位置 ted基因的范围已经缩小了Kale博士会找出潜在的ted 转录单位，分离那些RNA的cDNA克隆，并测序和 绘制TED基因。 最后，为了证明他确实克隆了泰德，凯尔博士 将ted基因组DNA插入到种系转化载体中， 确定DNA是否可以拯救突变。