ENHANCEMENT OF FACULTY RESEARCH CAPABILITY

加强教师研究能力

• 批准号: 6384926
• 负责人: PURUSHOTTAM G KALE
• 金额: $ 2.77万
• 依托单位: ALABAMA AGRI AND MECH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6384926
• 关键词: Drosophilidae; developmental genetics; gene mutation; genetic mapping; genetic transcription; molecular cloning; radiation genetics; southern blotting

DESCRIPTION: This MORE faculty development award is to provide Dr. Kale with training in molecular biology. Dr. Kale will spend five consecutive summers investigating the Drosophila ted gene, under the guidance and in the lab of Dr. Cavener. This gene function is needed for the adult fly to emerge from the pupal case. The approximate chromosomal position of ted is known. Genomic clones of this chromosomal region are available. To clone ted, the gene must be localized more precisely within this region. Dr. Kale will begin this by inducing ted mutations with X-rays, and then mapping the breakpoints of these lesions via Southern blotting. Once the position of ted gene has been narrowed down, Dr. Kale will identify potential ted transcription units, isolate cDNA clones for those RNAs, and sequence and map the ted gene. Finally, to prove that he has indeed cloned ted, Dr. Kale will insert ted genomic DNA into a germline transformation vector and determine whether the DNA can rescue ted mutations.

描述：本MORE教师发展奖旨在提供卡勒博士