MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

该提案要求为购买变速器提供资金 用于微血管研究的电子显微镜。主要用户 由七名调查员组成的小组，他们都在从事 NIH支持的研究活动，已确定。各自的项目 主要用户需要电子显微镜作为主要 研究工具或作为多学科研究方法的一部分 对微循环的影响。不能充分利用现有设备， 主要归因于1)大量的调查人员 目前正在争夺显微镜时间，2)优先注册 合同工作，再加上无法获得仪器 由于房间限制，在离线光谱分析期间，以及4) 机械故障，已经损害了我们满足 现有赠款承诺和延迟获取试点数据 关于使用电子显微镜的新建议。建议数 按要求配备的仪器将负担得起所需的 对所有调查人员可访问性，此外还提供直接 输入到数字化仪和计算机以进行图像分析/存储， 可加速数据采集和分析的功能 适用于许多应用程序。基于当前的需求，以及全面的 对显微镜的实际检验和理论检验 可供选择的JEOL JEM 1200EX已被选中。采购 电子显微镜的出现代表了5年来 旨在建立一支独立的、装备齐全的 微循环研究中的电子显微镜设备 研究所。该设施被认为是一个不可或缺的关键 该研究所长期计划的组成部分，以保证 多学科研究的持续增长和生产力 团队努力揭示治理的基本流程 中暑和疾病时的微循环行为。

This proposal requests funds for the purchase of a transmission electron microscope for microvascular research. A major user group comprising seven investigators, all of whom are engaged in NIH supported research activities, is identified. Projects of each major user require electron microscopy either as a primary research tool or as part of a multidisciplinary approach to studies of the microcirculation. inadequate access to existing equipment, attributed primarily to 1) the large number of investigators currently vying for microscope time, 2) priority sign-up given contract work, and compounded by 3) instrument inavailabfility during off-line spectral analysis due to room limitations, and 4) mechanical failure, has compromised both our ability to meet existing grant commitments and delayed acquisition of pilot data for new proposals employing electron microscopy. The proposed instrument, equipped as requested, would afford the desired accessibility to all investigators and in addition provide direct input to digitizer and computer for image analysis/storage, capabilities which would accelerate data acquisition and alalysis for many applications. Based on current needs, and a thorough examination, in practice as well as theory, of the microscopes available, the JEOL JEM 1200EX has been selected. Procurement of an electron microscope represents the final step in a 5-year plan directed at establishing an independent, fully-equipped electron microscopy facility in the Microcirculation Research Institute. The facility is considered an integral an crucial component of the institute's long range plan to guarantee continued growth and productivity of a multidisciplinary research team striving to uncover the fundamental processes governing microcirculatory behavior in heatlh and disease.