ENHANCEMENT OF FACULTY RESEARCH CAPABILITY

描述：这个更多的教师发展奖是为了向Kale博士提供 接受过分子生物学方面的训练。凯尔博士将连续五年 萨默斯研究果蝇Ted基因，在指导下和在 卡文纳博士的实验室。这种基因功能对于成年苍蝇来说是必要的 从蚕蛹案件中脱颖而出。TED的大致染色体位置是 为人所知。这个染色体区域的基因组克隆是可用的。克隆 泰德，基因必须更精确地定位在这个区域内。凯尔博士 将首先用X射线诱导Ted突变，然后绘制 Southern blotting显示这些损伤的断裂点。一旦…的位置 泰德基因已被缩小，凯尔博士将确定潜在的泰德 转录单位，分离这些RNA的cdna克隆，并测序和 绘制泰德基因图谱。最后，为了证明他确实克隆了泰德，凯尔博士 将TED基因组DNA插入到生殖系转化载体中 确定DNA是否可以挽救Ted突变。

DESCRIPTION: This MORE faculty development award is to provide Dr. Kale with training in molecular biology. Dr. Kale will spend five consecutive summers investigating the Drosophila ted gene, under the guidance and in the lab of Dr. Cavener. This gene function is needed for the adult fly to emerge from the pupal case. The approximate chromosomal position of ted is known. Genomic clones of this chromosomal region are available. To clone ted, the gene must be localized more precisely within this region. Dr. Kale will begin this by inducing ted mutations with X-rays, and then mapping the breakpoints of these lesions via Southern blotting. Once the position of ted gene has been narrowed down, Dr. Kale will identify potential ted transcription units, isolate cDNA clones for those RNAs, and sequence and map the ted gene. Finally, to prove that he has indeed cloned ted, Dr. Kale will insert ted genomic DNA into a germline transformation vector and determine whether the DNA can rescue ted mutations.