IDENTIFICATION OF NOVEL STAPHYLOCOCCAL VIRULENCE GENES

描述(摘自摘要)：金黄色葡萄球菌仍是 社区和医院发病率和死亡率的主要原因 设置。金黄色葡萄球菌的多种耐药菌株很常见， 我们距离回到抗生素前时代只有一种抗生素了，它的 随之而来的是高死亡率，感染这种细菌。它是 相信对细菌致病性的基础研究将提供 改进疾病控制方法的科学平台。上一首 金黄色葡萄球菌致病机制的研究日益受到重视。 由有机体产生的细胞外因子。蛋白质的作用 在细菌对寄主环境的压力下产生的 近年来，致病性越来越受到人们的重视。这项研究将 检验新的金黄色葡萄球菌应激诱导毒力因子的假设 并且相关的调控元件可以在mRNA的基础上分离出来 表情。基于聚合酶链式反应的方法称为 差异显示将用于检测mRNA表达的差异 以响应模拟宿主环境的压力。这些 研究可能导致确定抗菌剂的新靶点 探员们。

DESCRIPTION (Adapted from the Abstract): Staphylococcus aureus remains a leading cause of morbidity and mortality in both community and hospital settings. Multiple antibiotic resistant strains of s. aureus are common and we are one antibiotic away from returing to the pre antibiotic era, with its attendant high mortality rates, for infections with this bacterium. It is believed that fundamental studies of bacterial pathogenicity will provide the scientific platform for improved methods of disease control. Previous studies of the mechanisms of S. aureus pathogenicity have emphasized extracellular factors produced by the organism. The role of proteins produced in response to the stresses of the host environment in bacterial pathogenicity has been increasingly recognized of late. This study will examine the hypothesis that novel S. aureus stress-induced virulence factors and associated regulatory elements can be isolated on the basis of mRNA expression. The polymerase chain reaction based approach known as differential display will be used to detect differences in mRNA expression in response to stresses mimicking those of the host environment. These studies may result in the identification of novel targets for antimicrobial agents.