IDENTIFICATION OF NOVEL STAPHYLOCOCCAL VIRULENCE GENES

新型葡萄球菌毒力基因的鉴定

• 批准号: 2616875
• 负责人: BRIAN JAMES WILKINSON
• 金额: $ 9.6万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 2001-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2616875
• 关键词: Staphylococcus aureus; bacterial genetics; bacterial proteins; gene induction /repression; microorganism culture; nucleic acid sequence; polymerase chain reaction; site directed mutagenesis; stress proteins; virulence

DESCRIPTION (Adapted from the Abstract): Staphylococcus aureus remains a leading cause of morbidity and mortality in both community and hospital settings. Multiple antibiotic resistant strains of s. aureus are common and we are one antibiotic away from returing to the pre antibiotic era, with its attendant high mortality rates, for infections with this bacterium. It is believed that fundamental studies of bacterial pathogenicity will provide the scientific platform for improved methods of disease control. Previous studies of the mechanisms of S. aureus pathogenicity have emphasized extracellular factors produced by the organism. The role of proteins produced in response to the stresses of the host environment in bacterial pathogenicity has been increasingly recognized of late. This study will examine the hypothesis that novel S. aureus stress-induced virulence factors and associated regulatory elements can be isolated on the basis of mRNA expression. The polymerase chain reaction based approach known as differential display will be used to detect differences in mRNA expression in response to stresses mimicking those of the host environment. These studies may result in the identification of novel targets for antimicrobial agents.

描述（改编自摘要）：金黄色葡萄球菌仍然是一种 社区和医院发病率和死亡率的主要原因 设置. 多重抗生素耐药菌株。金黄色葡萄球菌很常见， 我们距离回到前抗生素时代只有一种抗生素， 伴随着高死亡率，因为感染了这种细菌。 是 相信对细菌致病性的基础研究将提供 为改进疾病控制方法提供科学平台。 先前 对S.金黄色葡萄球菌的致病性已经强调 由生物体产生的细胞外因子。 蛋白质的作用 在细菌中响应宿主环境的应激而产生 近来，致病性越来越被认识到。 本研究将 检验了新的S.金黄色葡萄球菌应激诱导毒力因子 和相关的调节元件可以基于mRNA分离 表情 基于聚合酶链反应的方法被称为 差异显示将用于检测mRNA表达的差异 以响应模仿宿主环境的压力。 这些 研究可能会导致确定新的目标， 剂.

项目成果

Molecular characterization of a chromosomal locus in Staphylococcus aureus that contributes to oxidative defence and is highly induced by the cell-wall-active antibiotic oxacillin.

金黄色葡萄球菌染色体位点的分子特征，该位点有助于氧化防御，并受到细胞壁活性抗生素苯唑西林的高度诱导。

• DOI: 10.1099/00221287-147-11-3037
• 发表时间: 2001
• 期刊: Microbiology (Reading, England)
• 作者: Singh,VK;Moskovitz,J;Wilkinson,BJ;Jayaswal,RK
• 通讯作者: Jayaswal,RK

Pine oil cleaner-resistant Staphylococcus aureus: reduced susceptibility to vancomycin and oxacillin and involvement of SigB.

耐松油清洁剂的金黄色葡萄球菌：对万古霉素和苯唑西林的敏感性降低以及 SigB 的参与。

• DOI: 10.1128/aem.68.11.5417-5421.2002
• 发表时间: 2002
• 期刊: Applied and environmental microbiology
• 影响因子: 4.4
• 作者: Price,ChristopherTD;Singh,VineetK;Jayaswal,RadheshyamK;Wilkinson,BrianJ;Gustafson,JohnE
• 通讯作者: Gustafson,JohnE