METAL REGULATED GENE EXPRESSION IN PROKARYOTES

原核生物中金属调控的基因表达

• 批准号: 2605163
• 负责人: SILVIA ROSSBACH
• 金额: $ 10.7万
• 依托单位: WESTERN MICHIGAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-21 至 2001-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2605163
• 关键词: Pseudomonas; bacterial genetics; cadmium; copper; gene induction /repression; heavy metals; microorganism culture; molecular cloning; reporter genes; transposon /insertion element; zinc

DESCRIPTION: (applicant's abstract) The overall goal of this proposal is to study the responses of cells to high concentrations of heavy metals. Certain metal ions, including those of copper and zinc, are essential for a variety of metabolic functions of the cell, and these ions have to be efficiently scavenged from the environment. On the other hand, organisms can be exposed to high concentrations of heavy metals ions through metabolic disorders, polluted environments, or metal contaminated food. Thus, cells have to protect themselves against the damage caused by toxic concentrations of heavy metal ions. Here, reporter gene fusion technology is employed to study metal dependent gene expression in a prokaryotic model system. A random mutagenesis protocol is used to generate a mutant bank of the bacterium Pseudomonas fluorescens with the lac Z-based reporter gene transposon Tn5-B20. By monitoring the lacZ encoded beta-galactosidase activity, genes responding to the presence of heavy metal ions will be identified and their expression in response to heavy metals analyzed. The hypothesis to be tested is that cells will respond to the exposure of toxic heavy metals, such as cadmium, with the expression of a specific set of genes. Some of these genes will be involved in the specific protection mechanism of the cell against metal ions, others will be part of the general stress response of the cell. The reaction of the cell to toxic cadmium ions will be compared with the response of the cell to the essential metal ions of copper and zinc.

描述：（申请人摘要）本提案的总体目标是

项目成果

Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens.

• DOI: 10.1111/j.1574-6968.2000.tb09320.x
• 发表时间: 2000-10
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: Silvia Rossbach;Thomas L. Wilson;M. L. Kukuk;Heather A Carty

Cadmium-regulated gene fusions in Pseudomonas fluorescens.

• DOI: 10.1046/j.1462-2920.2000.00117.x
• 发表时间: 2000-08
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: S. Rossbach;M. L. Kukuk;T. L. Wilson;S. Feng;M. Pearson;M. Fisher