ETHANOL NICOTINE INTERACTION IN ALCOHOL PREFERRING RATS

本申请涉及一项探索性国际项目的资金筹措， 合作研究遗传和神经生物学基础 酗酒行为。合作地点是印第安纳州 美国印第安纳波利斯大学酒精研究中心（IARC）， 和加拿大多伦多的成瘾研究基金会。 两者都建立了酒精研究人员谁将带来独特的 资源和专业知识，以这种合作的努力。 独特的 ARF为该项目带来的资源是精选的酒精- 偏好HARF和非偏好IARF大鼠品系， 基于有限饮用范式开发，以模仿 酗酒 IARC为该项目带来的独特资源是 高酒精偏好HAD和低酒精偏好LAD大鼠 根据24 h.自由选择（连续访问） 消耗10%乙醇。 重要的是，这两对选定的 品系来源于相同的异质原种N/Nih大鼠。 的 因此，合作是一个前所未有的机会， 比较和对比行为和神经生物学特征， 与两种不同的饮酒表型有关， 作为发现潜在共同机制的一种方式。 发起 合作，我们将解决酒精的重要问题- 尼古丁的相互作用，并使合作的实验室 资源和专业知识的威廉Corrigall，在研究的先驱 实验动物的尼古丁自我给药。 我们提出 研究以检查是否存在以下方面的品系差异：l） 尼古丁和乙醇对自发运动活动和身体的影响 温度; 2）对尼古丁作用的致敏作用 和乙醇对重复尼古丁后的自发活动的影响， 乙醇给药; 3）急性和慢性尼古丁的影响 预处理酒精消费;和4）收购率 和IV尼古丁自我给药的程度。

This application regards funding of an exploratory international collaboration to study the genetic and neurobiological underpinnings of alcohol-seeking behavior. The collaborating sites are the Indiana University Alcohol Research Center (IARC) in Indianapolis, USA, and the Addiction Research Foundation (ARF) in Toronto, Canada. Both have established alcohol researchers who will bring unique resources and expertise to this collaborative effort. The unique resource ARF brings to the project are the selectively-bred alcohol- preferring HARF and nonpreferring IARF rat lines that are being developed, based on the limited-access drinking paradigm to mimic binge drinking. The unique resource IARC brings to the project are the high alcohol-preferring HAD and low alcohol-preferring LAD rat lines, selected on the basis of 24 h. free-choice (continuous access) consumption of 10% ethanol. Importantly, both pairs of selected lines originate from the same heterogeneous stock N/Nih rat. The collaboration, therefore, represents an unprecedented opportunity to compare and contrast the behavioral and neurobiological traits that are associated with the two differently selected drinking phenotypes, as a way of uncovering potentially common mechanisms. To initiate the collaboration, we will address the important question of alcohol- nicotine interaction and bring to the collaboration the laboratory resources and expertise of William Corrigall, a pioneer in studying nicotine self-administration by the laboratory animal. We propose studies to examine if line differences exist in: l) the acute effects of nicotine and ethanol on spontaneous locomotor activity and body temperature; 2) development of sensitization to the effects of nicotine and ethanol on locomotor activity following repeated nicotine or ethanol administration; 3) the effects of acute and chronic nicotine pretreatment on alcohol consumption; and 4) the rate of acquisition and extent of IV nicotine self-administration.