NOVEL IMMUNO POLYUMER DEVICE FOR CD34+ CELL ISOLATION

用于 CD34 细胞分离的新型免疫聚合物装置

• 批准号: 2646459
• 负责人: MICHAEL John LAWMAN
• 金额: $ 10万
• 依托单位: MORPHOGENESIS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2646459
• 关键词: CD34 molecule; cell sorting; chemical synthesis; electrochemistry; hematopoietic stem cells; human tissue; immunoconjugates; method development; polymers

DESCRIPTION: (Adapted from the applicant's abstract) The purpose of the proposed research is to develop a new stem cell selection technology based on a conducting immuno-polymer. The immunopolymer solid matrix is synthesized using a simple polymerization reaction during which stem cell-specific antibodies (anti-CD34) are entrapped. The conductive properties of this matrix could improve or solve a variety of problems associated with the current cell selection technologies. In particular, its conductivity could be exploited to monitor and computer control cell binding during the selection process, to reduce non-specific binding, and to release the selected cells. The applicant proposes to optimize the conditions of synthesis of the matrix using direct and indirect entrapment of the antibodies in the polymer. The cell binding characteristics of the immuno-polymers will be assessed using hematopoietic cell lines. Different electrochemical strategies for the release of bound cells and their effects on cell viability will be tested. The clinical applications of this technology include stem cell selection, T-cell or tumor cell depletion, and residual tumor cell enrichment for improved detection. The long term goal is to develop a cell selection device capable of directly and efficiently processing whole peripheral blood or bone marrow for transplantation. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：(改编自申请人的摘要