MULTINUCLEAR NMR PROBES OF BIOLOGICAL SYSTEMS

生物系统的多核核磁共振探针

• 批准号: 2905207
• 负责人: IAN M ARMITAGE
• 金额: $ 19.34万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2905207
• 关键词: Malacostraca; Neurospora; chemical kinetics; heavy metals; intermolecular interaction; metal metabolism; metallothionein; nuclear magnetic resonance spectroscopy; protein isoforms; protein structure function

DESCRIPTION: This proposal has as its focus the structural and functional role of the ubiquitously distributed cysteine-rich metal binding protein metallothionein (MT). MT was discovered 38 years ago, but its physiological role remains unconfirmed. However, its biosynthesis is closely regulated by exposure to heavy metal ions and in combination with extensive experimental data on metal binding, has lead to a proposal that MT is a critical component in metal homeostasis. A central focus of this work will be to determine 3D solution structures of three MTs, one mammalian, one from Neurospora crassa and one from the blue Crab. In previous studies unique metal-ion lability has been demonstrated for the 3-metal cluster in beta domain. It was demonstrated that intermolecular exchange occurs between beta-sites, and another goal of this proposal is to elucidate the mechanistic and kinetic details of this metal exchange phenomenon.

描述：这项提案的重点是结构性和功能性 广泛分布的富含半胱氨酸的金属结合蛋白的作用 金属硫蛋白(MT)。MT是38年前发现的，但它的生理 角色仍未得到确认。然而，它的生物合成受到严格的 暴露于重金属离子，并结合广泛的实验 关于金属结合的数据，导致了一种建议，即MT是一个关键 金属动态平衡的成分。这项工作的一个中心重点将是 确定三种MT，一种哺乳动物，一种来自 粗毛脉孢菌和蓝蟹的一株。在以前的研究中独一无二 在测试版中，已经证明了3金属团簇的金属离子不稳定。 域。研究表明，分子间交换发生在分子间。 Beta站点，这项提案的另一个目标是阐明 这种金属交换现象的机理和动力学细节。

项目成果

Nuclear magnetic resonance spectroscopy of skin: predictive correlates for clinical application.

皮肤核磁共振波谱：临床应用的预测相关性。

• DOI: 10.1097/00006534-198801000-00001
• 发表时间: 1988
• 期刊: Plastic and reconstructive surgery
• 影响因子: 3.6
• 作者: Cuono,CB;Armitage,IM;Marquetand,R;Chapo,GA
• 通讯作者: Chapo,GA

Dynamics of interdomain and intermolecular interactions in mammalian metallothioneins.

哺乳动物金属硫蛋白结构域间和分子间相互作用的动力学。

• DOI: 10.1016/s0162-0134(01)00379-8
• 发表时间: 2002
• 期刊: Journal of inorganic biochemistry
• 影响因子: 3.9
• 作者: Zangger,Klaus;Armitage,IanM
• 通讯作者: Armitage,IanM

Proton NMR studies of a metallothionein from Neurospora crassa: sequence-specific assignments by NOE measurements in the rotating frame.

粗糙脉孢菌金属硫蛋白的质子核磁共振研究：通过旋转坐标系中的 NOE 测量进行序列特异性分配。

• DOI: 10.1021/bi00433a038
• 发表时间: 1989
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Malikayil,JA;Lerch,K;Armitage,IM
• 通讯作者: Armitage,IM

Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3.

• DOI: 10.1021/bi010827l
• 发表时间: 2001-09
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: G. Öz;K. Zangger;I. Armitage

Re-evaluation of the binding of ATP to metallothionein.

重新评估 ATP 与金属硫蛋白的结合。

• DOI: 10.1074/jbc.275.11.7534
• 发表时间: 2000
• 期刊: The Journal of biological chemistry
• 作者: Zangger,K;Oz,G;Armitage,IM
• 通讯作者: Armitage,IM