HIGH FIELD NMR SPECTROMETER FOR BIOLOGICAL STUDIES

用于生物学研究的高场核磁共振波谱仪

• 批准号: 3519797
• 负责人: IAN M ARMITAGE
• 金额: $ 30万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-01-15 至 1988-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519797
• 关键词: biology; chemical structure; macromolecule; nuclear magnetic resonance spectroscopy

Today, nuclear magnetic resonance is one of the most widely used tools for the detailed elucidation of the structure and dynamics of compounds of chemical and biological interest. In the last few years, the surge of interest in applications of NMR to problems in biology and medicine can be directly attributed to improvements in instrumental and magnet technology and the advent of various two-dimensional NMR methods. In order to accommodate the critical need for these facilities in the research programs of several primary investigators in the Yale School of Medicine, funds are requested in this application for a high field (11.75T) NMR spectrometer that will be housed in the new comprehensive NMR Center at the Yale School of Medicine and dedicated to macromolecular/biomedical NMR research. In support of this application are the research protocols of nine principal investigators from within the Yale School of Medicine requiring state-of-the-art high field NMR, in particular protons at 500 MHz, for the structural characterization of a variety of biologically important macromolecules, their substrates and related metabolic intermediates. Application of NMR in all of the areas proposed has only recently been made possible by the availability of NMR spectrometers operating at fields that are high enough to provide resolution of signals from individual sites within macromolecules or from individual metabolites in the complex mixtures that exist in various biological fluids or cell extracts. The projects outlined in this application cover a broad spectrum of active, ongoing research in biology and medicine for which the proposed use of high field NMR is both essential and not otherwise available.

今天，核磁共振是最广泛使用的 的结构和动力学的详细说明的工具 具有化学和生物学意义的化合物。 过去几 近年来，人们对核磁共振应用于解决 生物学和医学可以直接归因于 在仪器和磁铁技术和各种出现 二维NMR方法。 为了容纳 这些设施的研究计划的迫切需要， 耶鲁医学院的几位主要研究人员， 本申请要求资金用于高磁场（11.75T） NMR光谱仪，将被安置在新的综合 耶鲁医学院核磁共振中心，致力于 大分子/生物医学NMR研究。 支持这项申请的是九项研究协议， 耶鲁医学院的主要研究人员 需要最先进的高场NMR，特别是质子， 500 MHz，用于各种结构表征 生物学上重要的大分子，它们的底物， 相关代谢中间体。 核磁共振在所有领域的应用 建议的地区最近才得以实现， NMR光谱仪的可用性， 高到足以提供来自各个站点的信号的分辨率 在大分子内或从单个代谢物中 存在于各种生物液体或细胞中的复杂混合物 萃取物 本申请中概述的项目涵盖广泛的 在生物学和医学领域正在进行的一系列积极研究， 所提出的高场NMR的使用是必要的， 其他可用的。