PHYSICAL PROPERTIES OF LIPOPOLYSACCHARIDES

脂多糖是一种大分子两亲性物质，由uP组成。 至革兰氏阴性菌外膜重量的三分之一 细菌。内毒素在外周屏障功能中起着至关重要的作用 膜除了展示出大量的强效 免疫病理活动。我们建议使用多核核磁共振方法 来阐明这些分子的结构，以便在 分子水平上影响生物活性的因素 (内毒素和免疫原性)的内毒素。一个详细的 对膜的结构、金属离子结合和膜组织的理解 内毒素的各个方面是理解分子的基本前提。 内毒素在维持细菌完整性中的作用基础 外膜，在革兰氏阴性菌和ALL的发病机制中 努力合成内毒素的最小活性亚基。在……里面 结合适当的生物学研究，从 本文提出的核磁共振研究将有助于免疫系统的合理设计 反应改进剂，包括开发有效和安全的 免疫治疗剂。

Lipopolysaccharides (LPS) are macromolecular amphiphiles that comprise up to one third by weight of the outer membrane of the gram-negative bacteria. LPS plays a vital role in the barrier function of the outer membrane in addition to exhibiting a large number of potent immunopathalogical activities. We propose to use multinuclear NMR methods to elucidate the structure of these molecules in order to understand at the molecular level the factors responsible for the biological activities (endotoxic and immunogenic) of lipopolysaccharides. A detailed understanding of the structure, metal ion binding and membrane organization aspects of LPS is an essential prerequisite to understanding the molecular basis for the function of LPS in maintaining the integrity of the bacterial outer membrane, in the pathogenesis of gram negative bacteria and for all efforts to synthesize the minimal active subunit of the endotoxin. In conjunction with appropriate biological studies, the data obtained from the NMR studies proposed herein will lead to the rational design of immune response modifiers, including the development of effective and safe immunotherapeutic agents.