PHYSICAL PROPERTIES OF LIPOPOLYSACCHARIDES

脂多糖的物理性质

• 批准号: 3130872
• 负责人: IAN M ARMITAGE
• 金额: $ 5.4万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130872
• 关键词: Escherichia coli; Salmonella; Salmonella typhimurium; bacterial polysaccharides; bacterial somatic antigen; cadmium; calcium; cell membrane; chemical structure; conformation; endotoxins; ligands; lipopolysaccharides; magnesium; membrane reconstitution /synthesis; mutant; nuclear magnetic resonance spectroscopy; radiotracer

Lipopolysaccharides (LPS) are macromolecular amphiphiles that comprise up to one third by weight of the outer membrane of the gram-negative bacteria. LPS plays a vital role in the barrier function of the outer membrane in addition to exhibiting a large number of potent immunopathalogical activities. We propose to use multinuclear NMR methods to elucidate the structure of these molecules in order to understand at the molecular level the factors responsible for the biological activities (endotoxic and immunogenic) of lipopolysaccharides. A detailed understanding of the structure, metal ion binding and membrane organization aspects of LPS is an essential prerequisite to understanding the molecular basis for the function of LPS in maintaining the integrity of the bacterial outer membrane, in the pathogenesis of gram negative bacteria and for all efforts to synthesize the minimal active subunit of the endotoxin. In conjunction with appropriate biological studies, the data obtained from the NMR studies proposed herein will lead to the rational design of immune response modifiers, including the development of effective and safe immunotherapeutic agents.

脂多糖（LPS）是大分子两亲物， 革兰氏阴性菌外膜重量的三分之一 细菌 LPS在外周血淋巴细胞的屏障功能中起着至关重要的作用， 膜除了表现出大量的有效的 免疫病理活性。 我们建议使用多核核磁共振方法 来阐明这些分子的结构， 分子水平上负责生物活性的因子 （内毒素和免疫原性）的脂多糖。 详细 了解结构、金属离子结合和膜组织 LPS的各个方面是了解LPS分子的必要先决条件。 LPS在维持细菌完整性中的功能基础 外膜，在革兰氏阴性菌的发病机制和所有 努力合成内毒素的最小活性亚基。 在 结合适当的生物学研究，从 本文提出的NMR研究将导致免疫的合理设计， 反应调节剂，包括开发有效和安全的 免疫抑制剂。

项目成果

Selective detection of 3-deoxymannooctulosonic acid in intact lipopolysaccharides by spin-echo 13C NMR.

通过自旋回波 13C NMR 选择性检测完整脂多糖中的 3-脱氧甘露辛糖酸。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Strain,SM;Armitage,IM
• 通讯作者: Armitage,IM

Location of polar substituents and fatty acyl chains on lipid A precursors from a 3-deoxy-D-manno-octulosonic acid-deficient mutant of Salmonella typhimurium. Studies by 1H, 13C, and 31P nuclear magnetic resonance.

鼠伤寒沙门氏菌 3-脱氧-D-甘露-辛糖酸缺陷突变体脂质 A 前体上极性取代基和脂肪酰基链的位置。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Strain,SM;Armitage,IM;Anderson,L;Takayama,K;Qureshi,N;Raetz,CR
• 通讯作者: Raetz,CR