REGENERATION IN IMAGINAL DISCS OF DROSOPHILA

果蝇成虫盘的再生

• 批准号: 2910442
• 负责人: Gerold A. Schubiger
• 金额: $ 18.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2910442
• 关键词: Drosophilidae; biological signal transduction; blastema; cell growth regulation; cell proliferation; developmental genetics; gene expression; gene interaction; genetic manipulation; genetic mapping; genetic regulation; imaginal disc; limb regeneration; pluripotent stem cells; wound healing

DESCRIPTION (adapted from investigator's abstract): Classic experimental approaches to regeneration have provided insight into three conserved aspects of the process: wound healing, formation of a "blastema" consisting of pluripotent, highly proliferative cells, and pattern regulation. This study proposes to study these processes at the molecular level by mapping the expression patterns, and also manipulating the functions, of genes involved in patterning and cell cycle control in the leg primordia of Drosophila, called imaginal discs. The preliminary data provided shows that a central player in normal and regenerative limb development is the secreted signal wingless. Homologs of wingless have analogous function in vertebrate limb patterning and regeneration, such as wound healing, transplantation and chemotherapy. Studies in Drosophila are advantageous because the system has advanced tools for genetic manipulation. The first aim of this proposal is to provide a molecular description of the sequence of patterning gene expression and function during imaginal leg disc regeneration, and in so doing to define the roles wingless and other secreted signals such as dpp play in the process. The second aim is to identify which of the known cell cycle genes control cell proliferation in regeneration blastemas, and to link regulation of these genes to the patternin cues that promote blastema growth. The third aim is to identify new genes that interact with wingless during regeneration, using a novel genetic screen. The findings will allow specific working hypotheses to be developed pertaining to cell cycle control, wound healing and growth during regeneration.

描述(改编自研究人员摘要)：经典实验 再生的方法提供了对三个保守的 这一过程的方方面面：伤口愈合，形成由 多能、高度增殖的细胞，以及模式调节。这 这项研究建议通过作图在分子水平上研究这些过程 基因的表达模式，以及对其功能的调控 参与小腿原基的构型和细胞周期控制 果蝇，被称为想象盘。提供的初步数据显示， 在正常的和可再生的肢体发育中，一个核心角色是分泌的 无翼信号。无翼的同系物在脊椎动物中具有类似的功能 肢体模式和再生，如伤口愈合、移植和 化疗。对果蝇的研究是有利的，因为该系统具有 先进的基因操作工具。这项提议的第一个目的是 为了提供花纹基因序列的分子描述 假想小腿间盘再生过程中的表达和功能 定义角色的无翼和其他秘密信号，如DPP 在这个过程中发挥作用。第二个目标是识别哪个已知细胞 周期基因控制再生胚泡中的细胞增殖，并 将这些基因的调节与促进胚泡发育的模式蛋白信号联系起来 成长。第三个目标是识别与无翼相互作用的新基因。 在再生过程中，使用了一种新的遗传屏幕。这一发现将允许 关于细胞周期的具体工作假说有待开发 再生期间的控制、伤口愈合和生长。