REGENERATION IN IMAGINAL DISCS OF DROSOPHILA

描述（改编自研究者摘要）：经典实验 再生的方法提供了三个保守的见解 过程的方面：伤口愈合，形成"芽基"， 多能性，高度增殖的细胞，和模式调节。 这 一项研究建议通过绘制分子水平的图谱来研究这些过程。 基因的表达模式和功能 参与腿部原基的图案形成和细胞周期控制， 果蝇，称为成虫盘。 提供的初步数据显示， 在正常和再生肢体发育中的一个中心角色是分泌的 无翼信号 在脊椎动物中，无翅的同源物具有类似的功能 肢体模式和再生，如伤口愈合，移植和 化疗 在果蝇中进行研究是有利的，因为该系统具有 先进的基因操作工具 这项建议的第一个目的是 提供模式基因序列的分子描述， 表达和功能，在成虫腿盘再生过程中， 做定义角色无翼和其他分泌信号，如DPP 玩在这个过程中。 第二个目标是确定哪个已知细胞 周期基因控制再生胚基中的细胞增殖， 将这些基因的调节与促进芽基形成的模式线索联系起来 增长 第三个目标是确定与无翅的相互作用的新基因 在再生过程中，使用一种新的遗传筛选。 调查结果将允许 与细胞周期有关的具体工作假设有待开发 控制、伤口愈合和再生过程中的生长。

DESCRIPTION (adapted from investigator's abstract): Classic experimental approaches to regeneration have provided insight into three conserved aspects of the process: wound healing, formation of a "blastema" consisting of pluripotent, highly proliferative cells, and pattern regulation. This study proposes to study these processes at the molecular level by mapping the expression patterns, and also manipulating the functions, of genes involved in patterning and cell cycle control in the leg primordia of Drosophila, called imaginal discs. The preliminary data provided shows that a central player in normal and regenerative limb development is the secreted signal wingless. Homologs of wingless have analogous function in vertebrate limb patterning and regeneration, such as wound healing, transplantation and chemotherapy. Studies in Drosophila are advantageous because the system has advanced tools for genetic manipulation. The first aim of this proposal is to provide a molecular description of the sequence of patterning gene expression and function during imaginal leg disc regeneration, and in so doing to define the roles wingless and other secreted signals such as dpp play in the process. The second aim is to identify which of the known cell cycle genes control cell proliferation in regeneration blastemas, and to link regulation of these genes to the patternin cues that promote blastema growth. The third aim is to identify new genes that interact with wingless during regeneration, using a novel genetic screen. The findings will allow specific working hypotheses to be developed pertaining to cell cycle control, wound healing and growth during regeneration.