REGENERATION IN IMAGINAL DISCS OF DROSOPHILA

果蝇成虫盘的再生

• 批准号: 2706772
• 负责人: Gerold A. Schubiger
• 金额: $ 23.43万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2706772
• 关键词: Drosophilidae; biological signal transduction; blastema; cell growth regulation; cell proliferation; developmental genetics; gene expression; gene interaction; genetic manipulation; genetic mapping; genetic regulation; imaginal disc; limb regeneration; wound healing

DESCRIPTION (adapted from investigator's abstract): Classic experimental approaches to regeneration have provided insight into three conserved aspects of the process: wound healing, formation of a "blastema" consisting of pluripotent, highly proliferative cells, and pattern regulation. This study proposes to study these processes at the molecular level by mapping the expression patterns, and also manipulating the functions, of genes involved in patterning and cell cycle control in the leg primordia of Drosophila, called imaginal discs. The preliminary data provided shows that a central player in normal and regenerative limb development is the secreted signal wingless. Homologs of wingless have analogous function in vertebrate limb patterning and regeneration, such as wound healing, transplantation and chemotherapy. Studies in Drosophila are advantageous because the system has advanced tools for genetic manipulation. The first aim of this proposal is to provide a molecular description of the sequence of patterning gene expression and function during imaginal leg disc regeneration, and in so doing to define the roles wingless and other secreted signals such as dpp play in the process. The second aim is to identify which of the known cell cycle genes control cell proliferation in regeneration blastemas, and to link regulation of these genes to the patternin cues that promote blastema growth. The third aim is to identify new genes that interact with wingless during regeneration, using a novel genetic screen. The findings will allow specific working hypotheses to be developed pertaining to cell cycle control, wound healing and growth during regeneration.

描述（改编自研究者的摘要）：经典实验 再生方法提供了对三种保守的见解 该过程的各个方面：伤口愈合，形成“胚基” 多能、高度增殖细胞和模式调节。 这 研究建议通过绘图在分子水平上研究这些过程 基因的表达模式以及操纵功能 参与腿部原基的模式形成和细胞周期控制 果蝇，称为成虫盘。 提供的初步数据表明 正常和再生肢体发育的核心参与者是分泌的 信号无翼。 wingless 的同源物在脊椎动物中具有类似的功能 肢体模式和再生，例如伤口愈合、移植和 化疗。 果蝇研究是有利的，因为该系统具有 基因操作的先进工具。 该提案的第一个目标是 提供模式基因序列的分子描述 成腿椎间盘再生过程中的表达和功能，以及因此 定义无翼角色和其他秘密信号（例如 dpp） 玩的过程中。 第二个目标是确定哪些已知细胞 周期基因控制再生胚基中的细胞增殖，并 将这些基因的调节与促进芽基的模式线索联系起来 生长。 第三个目标是识别与无翅相互作用的新基因 在再生过程中，使用一种新颖的基因筛选。 研究结果将允许 与细胞周期有关的具体工作假设 再生过程中的控制、伤口愈合和生长。