Regeneration of Imaginal Discs in Drosophila

果蝇成虫盘的再生

• 批准号: 6577438
• 负责人: Gerold A. Schubiger
• 金额: $ 28.56万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6577438
• 关键词: Drosophilidae; biological signal transduction; blastema; cell cycle; cell differentiation; cell growth regulation; cytogenetics; epithelium; flow cytometry; gene expression; imaginal disc; in situ hybridization; insulin receptor; regeneration; wound healing

DESCRIPTION (provided by applicant): The focus of the proposed experiments is to investigate cell signaling mechanisms between cell layers during organogenesis. Our previous work has shown that the columnar cells and the peripodial ceils in imaginal discs of Drosophila both grow in precise proportion to each other, suggesting coordinated regulation by a global size control mechanism. The first specific aim of this proposal is to provide a molecular description of the signaling pathways between the two cell layers. Studies with Drosophila imaginal discs are advantageous because upon identification of a pathway, we can remove or activate components of the pathway. This will tell us whether the pathway is only used for size control or organ identity or both. Based on preliminary data we focus our study on the wg, dpp, hh, and insulin signaling pathways between the two cell layers. In Drosophila, just as in other organisms, upon the wounding of an organ, wound healing occurs. A cut through an imaginal disc brings two cell layers together, and the two cell layers fuse shortly afterwards. The second aim of the proposal examines whether the molecular signals identified in the first specific aim also function in regeneration after wound healing. Preliminary observations indicate that wingless induces fate map changes necessary for regeneration. An understanding of how molecular signaling increases developmental potency during regeneration is key for stem cell biology.

描述(申请人提供)：拟议的实验的重点是研究器官发生过程中细胞层之间的细胞信号机制。我们之前的工作表明，果蝇成像盘中的柱状细胞和足周细胞都以精确的比例生长，这表明全球大小控制机制的协调调节。这项提议的第一个具体目标是提供两个细胞层之间的信号通路的分子描述。对果蝇成像盘的研究是有利的，因为一旦识别出一条途径，我们就可以移除或激活该途径的组成部分。这将告诉我们，这条途径是否只用于大小控制或器官识别，或者两者兼而有之。基于初步数据，我们重点研究了两个细胞层之间的WG、DPP、HH和胰岛素信号通路。在果蝇身上，就像在其他有机体中一样，器官受到损伤后，伤口就会愈合。切开想象中的盘将两个细胞层结合在一起，不久之后这两个细胞层融合在一起。该提案的第二个目的是检查在第一个特定目的中确定的分子信号是否也在伤口愈合后的再生中发挥作用。初步观察表明，无翼导致了再生所必需的命运图变化。理解分子信号如何在再生过程中增加发育潜力是干细胞生物学的关键。