ELECTROSTATIC SAMPLING OF AIRBORNE MICROORGANISMS

空气中微生物的静电采样

• 批准号: 2863109
• 负责人: KLAUS WILLEKE
• 金额: $ 19.6万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2863109
• 关键词: agriculture; air sampling /monitoring; biomedical equipment development; electric field; microorganism population study; occupational health /safety

DESCRIPTION: (Adapted from the Investigator's Abstract) Exposure to airborne microorganisms in agricultural, industrial, indoor and health-care environments causes over 100 million episodes per year of respiratory infections in the US. Side-by-side monitoring by different commercially available bioaerosols samplers does not result in the same data for a given exposure level because the viability of the organisms is affected by the stresses they are exposed to during the process of collection from the air environment. The overall objective of this research is to use electrostatic mechanisms to collect the airborne microorganisms, thus collecting the organisms more gently than is currently achievable by the inertial forces in conventional impactors and impingers. The collection medium will be a moving fluid that surrounds the collected organisms and moves them out of the electric field, thus minimizing losses in viability due to the electric field and avoiding desiccation during the sampling period. To find the optimal conditions for high efficiency collection with the least amount of viability loss, a bioaerosol generator with induction charging and no ozone production will be designed, built and used to study the effect of different charge levels on the organisms during collection. The electric field in the electrostatic precipitation unit will be varied from a high positive kilovolt/cm level to its equivalent negative value. The results will be evaluated relative to the naturally present charge levels embedded in the organisms. The electrostatic precipitation plates will be at an angle to the horizontal, and a continuous flow of fluid over the lower plate will remove the organisms from the electric field into a liquid pool where the organisms are filtered out while remaining in the liquid. The new sampler will be tested in the laboratory and in an agricultural environment with high concentrations of bioaerosols present. Comparison sampling will be performed against a conventional impactor and a conventional impinger. Analysis will be made on how to use the new collection method in indoor and health-care environments where the bioaerosol concentration is low.

描述：（改编自研究者摘要） 农业、工业、室内和卫生保健中的空气微生物 环境导致每年超过1亿次呼吸道感染 在美国感染。 由不同的商业机构进行并排监测 现有的生物气溶胶采样器不会产生相同的数据， 暴露水平，因为生物体的生存能力受到 在从空气中收集的过程中， 环境 本研究的总体目标是利用静电 收集空气中微生物的机制，从而收集 生物体更温和地比目前所能实现的惯性力， 传统的撞击器和撞击器。 收集介质将是 流动的液体包围着收集到的有机体， 电场，从而最大限度地减少由于电场引起的生存能力损失 在取样期间避免干燥。 找到 最佳条件下的高效率收集与最少量的 生存力损失，具有感应充电和无臭氧的生物气溶胶发生器 生产将被设计，建造和使用，以研究不同的影响， 收集过程中微生物的电荷水平。 中的电场 静电除尘装置将从一个高的积极变化 千伏/厘米级至其等效负值。 结果将 相对于天然存在的电荷水平， 有机体 静电除尘板将与 水平的，并且流体在下板上的连续流动将 将生物体从电场中移到液体池中， 生物体被滤出，同时保留在液体中。 新的采样器 将在实验室和农业环境中进行测试， 高浓度的生物气溶胶存在。 比较抽样将是 对传统的冲击器和传统的撞击器进行。 分析了新的采集方法在室内、 生物气溶胶浓度低的卫生保健环境。