ELECTROSTATIC SAMPLING OF AIRBORNE MICROORGANISMS

空气中微生物的静电采样

• 批准号: 6260509
• 负责人: KLAUS WILLEKE
• 金额: $ 21.76万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6260509
• 关键词: air sampling /monitoring; biomedical equipment development; electric field; microorganism population study; occupational health /safety; spores

DESCRIPTION: Each year millions of respiratory allergies and infections are caused by airborne microorganisms present in agricultural, industrial and indoor environments. The level of exposure indicated by bioaerosol samplers depends on the instrument used and the sensitivity of the microorganisms. In an effort to collect such microorganisms more gently, at low power and at minimal pressure drop, an electrostatic sampling technique has been developed and evaluated in the laboratory under the present NIOSH grant. As a major part of this development, an electrostatic particle-size classifier and a microorganism dispersion device with optional induction charging were developed to study the electric charges on airborne microorganisms. It has experimentally been proven that laboratory-dispersed indoor air bacteria, such as Pseudomonas fluorescens, had a net negative charge. Some of the bacteria were found to carry several thousand negative or positive charges. In contrast, particles of non-biological origin were found to carry very few positive or negative charges. This finding suggests that the electrostatic sampler will be capable of retaining airborne microorganisms by its electrostatic collecting field without first charging the microorganisms in the inlet section, thus reducing the complexity and power consumption for sampling in occupational environments. During the two years of the proposed grant continuation, this discovery will be evaluated in the laboratory with common bacteria and fungal spores and in the field through sampling of (1) microorganisms present in indoor air environments, (2) liquid-borne microorganisms in metalworking fluid environments, and (3) high concentrations of airborne microorganisms in agricultural environments. The physical and biological collection efficiencies will be determined from the relationship between the total and viable microorganisms collected by the new sampler and the number of uncollected microorganisms passed through the new sampler and collected by other means. The testing will indicate under what release conditions the microorganisms are sufficiently charged for collection by the simplified version of the new sampler. Microorganisms dispersed by mechanical action are expected to carry a particularly high charge and to be collected very efficiently by the electrostatic sampler without having to charge them in the inlet section. The new method will be used in occupational environments where airborne microorganisms are naturally present or are released by industrial processes.

产品说明： 每年数以百万计的呼吸道过敏和感染是由 存在于农业、工业和室内的空气微生物 环境.生物气溶胶采样器指示的接触水平取决于 使用的仪器和微生物的灵敏度。为了努力 在低功率和最小压力下更温和地收集这些微生物 滴，静电采样技术已开发和评估， 实验室在目前的NIOSH赠款。作为其中的主要部分， 发展，静电粒度分级机和微生物 分散装置与可选的感应充电，开发研究 空气中微生物的电荷。 实验证明，实验室分散的室内空气 细菌如荧光假单胞菌具有净负电荷。一些 发现细菌带有数千个负电荷或正电荷。 相比之下，非生物来源的颗粒被发现携带很少的 正电荷或负电荷。这一发现表明，静电 采样器将能够保留空气中的微生物， 静电收集场，而不首先使微生物带电， 入口部分，从而降低了采样的复杂性和功耗 在职业环境中。 在拟议的赠款延续的两年内，这一发现将被 在实验室中用常见的细菌和真菌孢子进行评价， 通过采样（1）室内空气中存在的微生物 环境，（2）金属加工液中的液体微生物 环境，和（3）高浓度的空气中的微生物 农业环境。物理和生物收集效率 将从总量和可行性之间的关系来确定 新采样器采集的微生物数量和未采集的数量 微生物通过新的采样器并通过其他方式收集。 检测将表明微生物在何种放行条件下 新的简化版的收集足够的收费 取样器。通过机械作用分散的微生物预计将携带 特别高的电荷，并被非常有效地收集， 静电采样器，而不必在入口部分对其充电。的 新方法将用于职业环境， 微生物是天然存在的或通过工业过程释放的。