MR DIAGNOSIS OF MUSCLE METABOLISM AND FUNCTION

描述：该项目的目标是开发诊断程序 用31-P/1-H核磁共振波谱和1-H核磁共振对人体肌肉功能的影响 成像与能量平衡原理一起在第一个 授权期。申请者工作假说是 能量平衡提供了定义正常所必需的机械性信息 并解释完好无损的人体四肢的异常肌肉功能。这个 前三个具体目标发展了综合质量的概念和 能量平衡。聚合酶链式反应含量衡量供需平衡 肌肉ATPase和氧化磷酸化。肌红蛋白脱饱和度 衡量氧化磷酸化和氧化磷酸化之间的供需平衡 肌肉灌流。细胞内pH和乳酸测量两者之间的平衡 净糖酵解通量和灌流洗脱。中国的收缩经济 人类四肢肌肉将通过对ATPase的特性进行量化，因为 肌纤维力的产生和兴奋-收缩耦合。 运动时的聚合酶链式反应水平是否是局部灌注量与氧分压的联系 需求将受到测试，其规模和时间进程 运动中的糖原分解流量将被量化。第四个具体问题 AIM将调查一个或多个失衡的两个临床问题 肌肉能量学的组成部分。充血性心力衰竭患者 将调查ATP、H和氧平衡的异常以及患者 1型糖尿病患者将在异常情况下进行权衡 导致肌肉功能降低，但接近正常的整合 能量学。

DESCRIPTION: The goal of this project is to develop diagnostic procedures for human muscle function using 31-P/1-H NMR spectroscopy and 1-H NMR imaging together with principles of energy balance developed in the first grant period. The applicants working hypothesis is that integration of energy balance provides mechanistic information essential to define normal and to interpret abnormal muscle function in the intact human limb. The first three specific aims develop the notions of an integrative mass and energy balance. PCr content measures the supply-demand balance between muscle ATPases and oxidative phosphorylation. Myoglobin desaturation measures the supply-demand balance between oxidative phosphorylation and muscle perfusion. Intracellular pH and lactate measures the balance between net glycolytic flux and washout by perfusion. The contractile economy in human limb muscles will be quantified by characterizing the ATPase due to myofibrillar force generation and to excitation-contraction coupling. Whether PCr level during exercise is the link between local perfusion and O2 demand will be tested and the magnitude and time course of the glycogenolytic flux during exercise will be quantified. The fourth specific aim will probe two clinical problems for imbalances in one or more components of muscle energetics. Patients with congestive heart failure will be probed for abnormalities in ATP, H+ and oxygen balances and patients with type 1 diabetes will be probed for a trade-off in abnormalities resulting in lower functional, but near-normal integration of muscle energetics.