Bruker Avance III 600 MHz (14T) Wide Bore Spectrometer

Bruker Avance III 600 MHz (14T) 宽口径光谱仪

• 批准号: 7840575
• 负责人: Martin J Kushmerick
• 金额: $ 101.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-10 至 2011-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7840575
• 关键词: Affect; Age; Aging; Anesthesiology; Apoptosis; Applications Grants; Basic Science; Biochemical; Biological; Biology of Aging; Cancer Biology; Cell physiology; Cells; Chronic; Commit; Data; Diagnostic; Diagnostic Procedure; Disease; Equipment; Faculty; Functional disorder; Funding; Grant; Heart failure; Image; Magnetic Resonance; Medicine; Metabolic; Metabolism; Methods; Minor; Mitochondria; Molecular; Mus; Myocardial; Myocardium; Natural regeneration; Pain; Physiological; Play; Productivity; Quality Control; Radiology Specialty; Recruitment Activity; Research; Research Personnel; Resolution; Resources; Role; Smooth Muscle Myocytes; Source; Stem cells; Systemic disease; Techniques; Testing; Translating; Tumor Biology; Universities; Washington; clinical application; heart metabolism; innovation; insight; instrument; malignant muscle neoplasm; medical schools; metabolomics; molecular imaging; mouse model; muscle aging; muscle metabolism; oxidative damage; programs; public health relevance; research study; response; tool

DESCRIPTION (provided by applicant): The fundamental purpose underlying this request for the purchase of a 600 MHz wide bore 14T imaging spectrometer is to provide an innovative research group with a unique and powerful new tool that will provide a key for advancing its research programs beyond current limitations. This new instrument will make new experiments possible and enhance the productivity of a number of R01 projects studying cellular physiology in heart, muscle, cancer and the biology of aging, with emphases on mitochondrial function in intact cells. The University of Washington has established a Mitochondria and Metabolism Center at a newly built facility of the School of Medicine in Seattle, located at the South Lake Union campus with newly recruited faculty. This group is forming a unique resource, co-directed by Drs. Kushmerick and Tian and established by the cooperation of, and jointly administered by, the Departments of Anesthesiology and Pain Medicine and of Radiology in the School of Medicine and the Provost's Office. The proposed high field NMR spectrometer and micro-imager will be a primary technological focus of the Center and a unique instrument in the School of Medicine and at the University of Washington. The field of "mitochondrial medicine" is expanding in importance. Both normal function and dysfunction of mitochondria play a central role in apoptosis and response to oxidative damage in addition to their role as the main source of metabolic energy in the cell. Innovative methodological and physiological advances developed by the users' grant projects have revealed insights into these mechanisms and clues for clinical applications. For example, with the addition of this high field spectrometer, we will be able to obtain critical functional data on mitochondrial function by magnetic resonance methods in conjunction with a wide range of biophysical, biochemical, physiological and molecular biological methods resident in the labs of our users. The facility will be maintained and quality control provided by MR expertise in the Department of Radiology and managed by both departments. The group of committed users includes well-funded projects in cardiac metabolism (Dr. Rong Tian), muscle metabolism and energetics (Drs. David Marcinek and Martin Kushmerick), aged muscle studies (Drs. Marcinek and Rabinovitch) and mouse tumor biology and molecular imaging (Dr. Donghoon Lee). In addition we have well-funded collaborators who are developing NMR techniques for their grants in myocardial stem cell and regeneration (Dr. Charles Murry), in mouse models of aging (Dr. Peter Rabinovitch), in cultured smooth muscle cells (Dr. Cecilia Giachelli) and in metabolomics (Dr. David Eaton). The proposed 14T instrument will be shared with those investigators and others within the University. PUBLIC HEALTH RELEVANCE: The research programs of the major and minor users of the magnetic resonance spectrometer proposed in this high-end instrument grant application encompass a wide scope of projects focused on furthering our understanding of and advancing diagnostic approaches for a range of disorders and diseases, including heart failure, aging, chronic and systemic diseases affecting muscle, and cancer. The equipment proposed is a very high resolution NMR spectrometer and microimager that will be used for testing hypotheses in basic science and serve to validate methods that can be translated into clinical applications and diagnostic procedures. The purchase of this equipment will allow these programs to advance in new and exciting directions, due to the advanced capabilities of this spectrometer.

描述（由申请人提供）：购买 600 MHz 宽口径 14T 成像光谱仪的基本目的是为创新研究小组提供独特而强大的新工具，为突破当前限制推进其研究项目提供关键。这种新仪器将使新的实验成为可能，并提高许多 R01 项目的生产力，这些项目研究心脏、肌肉、癌症和衰老生物学的细胞生理学，重点是完整细胞中的线粒体功能。华盛顿大学在西雅图医学院新建的设施中建立了线粒体和代谢中心，该设施位于南湖联合校区，并配备了新招募的教员。该小组正在形成一个独特的资源，由博士共同领导。该中心由 Kushmerick 和 Tian 共同创建，由医学院麻醉科、疼痛医学科以及放射科和教务长办公室合作建立并共同管理。拟议的高场核磁共振波谱仪和显微成像仪将成为该中心的主要技术焦点，也是医学院和华盛顿大学的独特仪器。 “线粒体医学”领域的重要性正在不断扩大。线粒体的正常功能和功能障碍除了作为细胞代谢能量的主要来源外，还在细胞凋亡和氧化损伤反应中发挥着核心作用。用户资助项目开发的创新方法和生理学进展揭示了对这些机制的见解和临床应用的线索。例如，通过添加这种高场光谱仪，我们将能够通过磁共振方法结合用户实验室中的各种生物物理、生物化学、生理和分子生物学方法来获取线粒体功能的关键功能数据。该设施将由放射科的 MR 专业人士进行维护和质量控制，并由两个部门管理。这群忠诚的用户包括心脏代谢（田荣博士）、肌肉代谢和能量学（David Marcinek 博士和 Martin Kushmerick 博士）、老年肌肉研究（Marcinek 和 Rabinovitch 博士）以及小鼠肿瘤生物学和分子成像（Donghoon Lee 博士）等领域资金充足的项目。此外，我们还有资金雄厚的合作者，他们正在开发 NMR 技术，以获得心肌干细胞和再生（Charles Murry 博士）、小鼠衰老模型（Peter Rabinovitch 博士）、培养平滑肌细胞（Cecilia Giachelli 博士）和代谢组学（David Eaton 博士）方面的资助。拟议的 14T 仪器将与这些研究人员和大学内的其他人共享。 公共健康相关性：本次高端仪器拨款申请中提出的磁共振波谱仪主要和次要用户的研究计划涵盖了广泛的项目，重点是加深我们对一系列疾病的理解和推进诊断方法，包括心力衰竭、衰老、影响肌肉的慢性和全身性疾病以及癌症。拟议的设备是一种非常高分辨率的核磁共振波谱仪和显微成像仪，将用于测试基础科学中的假设，并用于验证可转化为临床应用和诊断程序的方法。由于该光谱仪的先进功能，购买该设备将使这些项目朝着新的、令人兴奋的方向前进。