MODEL OF INTRACELLULAR BIOENERGETICS & ENERGY BALANCE

细胞内生物能量模型

• 批准号: 6119764
• 负责人: Martin J Kushmerick
• 金额: --
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-16 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119764
• 关键词: Mammalia; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical resource; human tissue; model design /development; positron emission tomography; technology /technique

Muscle provides a unique opportunity for quantifying a bioenergetic and energy balance system in living tissue. This study provides a special opportunity in this Resource to evaluate the mechanisms underlying intracellular chemical fluxes using a parallel approach combining experimental and modeling studies. The muscle system can be characterized as follows. Muscle activation leads to increase of cellular ATPases by more than 10-fold compared to the resting state. This increased ATP breakdown is balanced by ATP synthesis from the metabolic products of contraction as well as the activation signal (i.e., Ca++). These signals activate mitochondrial oxidative phosphorylation, which is the predominant process (>95%) driving the ATP resynthesis needed to attain energy balance. Our measurements of the relevant intracellular metabolites provide quantitative tests of how these sub-systems are organized and integrated to achieve energy balance. This project will develop quantitative models based on known enzyme and organelle properties and mechanisms. Our experimental systems provide tests of the kinetics and magnitudes of the chemical changes and fluxes predicted by these models. The result will be a comprehensive model of intracellular energetics applicable to striated muscle in general.

肌肉为量化 活组织中的生物能量和能量平衡系统。本研究 在本资源中提供了一个特殊机会来评估 细胞内化学通量的潜在机制 实验研究与模型研究相结合的方法。肌肉 系统可以有以下几个特点。肌肉激活导致 细胞内ATPase的增加比 休息状态。这种增加的ATP分解由ATP平衡 从收缩的代谢产物合成以及 激活信号(即，钙离子)。这些信号激活线粒体 氧化磷酸化，这是主要的过程(&gt；95%) 推动达到能量平衡所需的ATP再合成。我们的 对相关细胞内代谢产物的测量提供了 对这些子系统的组织方式进行量化测试 综合考虑，实现能源平衡。这个项目将会发展 基于量化模型的 已知的酶和细胞器的性质和机制。我们的 实验系统提供了动力学和大小的测试 这些模型所预测的化学变化和通量。结果 将是一个适用于细胞内能量学的综合模型 一般都是横纹肌。