MODEL OF INTRACELLULAR BIOENERGETICS & ENERGY BALANCE

肌肉提供了一个独特的机会， 生物能量和能量平衡系统。 本研究 在本资源中提供了一个特殊的机会来评估 细胞内化学通量的潜在机制， 方法结合实验和建模研究。 肌肉 系统的特点如下。 肌肉激活导致 细胞ATP酶的增加超过10倍， 静止状态 这种增加的ATP分解被ATP平衡 从收缩的代谢产物合成以及 激活信号（即，Ca++）。 这些信号激活线粒体 氧化磷酸化，这是主要过程（>95%） 驱动ATP的再合成以达到能量平衡。 我们 相关细胞内代谢物的测量提供了 对这些子系统如何组织的定量测试， 以达到能量平衡。 该项目将开发 定量模型 已知的酶和细胞器的特性和机制。 我们 实验系统提供了动力学和大小的测试， 这些模型预测的化学变化和通量。 结果 将是一个全面的细胞内能量学模型， 横纹肌的肌肉组织

Muscle provides a unique opportunity for quantifying a bioenergetic and energy balance system in living tissue. This study provides a special opportunity in this Resource to evaluate the mechanisms underlying intracellular chemical fluxes using a parallel approach combining experimental and modeling studies. The muscle system can be characterized as follows. Muscle activation leads to increase of cellular ATPases by more than 10-fold compared to the resting state. This increased ATP breakdown is balanced by ATP synthesis from the metabolic products of contraction as well as the activation signal (i.e., Ca++). These signals activate mitochondrial oxidative phosphorylation, which is the predominant process (>95%) driving the ATP resynthesis needed to attain energy balance. Our measurements of the relevant intracellular metabolites provide quantitative tests of how these sub-systems are organized and integrated to achieve energy balance. This project will develop quantitative models based on known enzyme and organelle properties and mechanisms. Our experimental systems provide tests of the kinetics and magnitudes of the chemical changes and fluxes predicted by these models. The result will be a comprehensive model of intracellular energetics applicable to striated muscle in general.