GRADIENT AND RF COIL UPGRADES FOR 47 T IMAGING SPECTROMETER: MUSCLE

47 T 成像光谱仪的梯度和 RF 线圈升级：肌肉

• 批准号: 7335277
• 负责人: Martin J Kushmerick
• 金额: $ 10.9万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335277
• 关键词: bioenergetics; brain; human; lead; muscle; neoplasm /cancer; obesity; perfusion; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The continued productivity of three leading research groups in Muscle Biology, Obesity and Cancer Biology require major improvements in imaging gradients, RF coils and physiologic gating and monitoring. The availability of a short bore 4.7 T Bruker magnet catalyzed the productivity of an integrated program of muscle studies using optical and MR spectroscopy to analyze metabolic and energy balances in human and animal muscles. New developments integrate optical NIR spectroscopy with MR in the investigation of mitochondrial dysfunction and integrate MR perfusion methods with spectroscopic measurements of metabolic fluxes to address important mechanisms in a range of maladies affecting muscle. Recent opportunities with collaborators elsewhere at this University enabled spectroscopic imaging of rat brain that measures molecules involved in cellular mechanisms regulating feeding and obesity. Further opportunities exist by combining MR and PET high resolution mouse imaging and in high resolution mouse imaging by leading cancer biology programs in our department and at the Fred Hutchinson Cancer Research Center. Research leadership by all of these NIH projects is jeopardized by inadequate gradients and RF coils and by the absence of tools for monitoring the physiological status of the animals in the magnet and for gating. This application requests funds to purchase a customized shim and gradient coils for this magnet, a very high gradient strength insert for rat and mouse studies, animal monitoring equipment and RF coils. The requested upgrades to our instrument will provide needed flexibility and capabilities for our twelve currently funded projects in the departments of Radiology, and Medicine at the University and at the Fred Hutchinson Cancer Research Center. All of these programs are internationally known for excellence in their fields. Human limb muscle bioenergetics, biomechanics and perfusion studies (Kushmerick, Conley, Marcinek and Marro) will continue their novel studies with this very high field instrument with clear-cut improvements in spatial resolution and signal to noise and in the ability to exploit novel multinuclear and multispectral (optics and NMR) methods. Quantification of metabolites in brain thought to be involved in regulation of feeding and obesity (Cummings) will for the first time be monitored dynamically during acute experiments and longitudinally in long term studies. High resolution 1H and 23Na imaging of liver, brain, prostate and implanted tumors will be facilitated for the studies of normal and transgenic mice (Sze and Miyaoka at UW and Grady, Kemp and Greenberg at FHCRC). The budget for this upgrade is modest for the large scope of projects being done.

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：肌肉生物学，肥胖和癌症生物学三个领先的研究小组的持续生产力需要在成像梯度，射频线圈和生理门控和监测方面进行重大改进。短孔4.7 T布鲁克磁铁的可用性促进了使用光学和磁共振光谱分析人类和动物肌肉代谢和能量平衡的肌肉研究综合计划的生产力。新的发展将光学近红外光谱与磁共振结合起来研究线粒体功能障碍，并将磁共振灌注方法与代谢通量的光谱测量结合起来，以解决一系列影响肌肉疾病的重要机制。最近与这所大学其他地方的合作者合作的机会使大鼠脑的光谱成像能够测量参与调节进食和肥胖的细胞机制的分子。进一步的机会存在于MR和PET高分辨率小鼠成像的结合，以及我们系和Fred Hutchinson癌症研究中心领先的癌症生物学项目的高分辨率小鼠成像。所有这些NIH项目的研究领导地位都受到了不充分的梯度和射频线圈以及缺乏监测磁铁和门控中动物生理状态的工具的威胁。该申请要求资金购买该磁铁的定制垫片和梯度线圈，用于大鼠和小鼠研究，动物监测设备和射频线圈的非常高的梯度强度插入。要求对我们的仪器进行升级，将为我们目前资助的12个项目提供所需的灵活性和能力，这些项目分别在大学放射科、医学系和弗雷德·哈钦森癌症研究中心。所有这些项目都以其在各自领域的卓越表现而闻名于世。人体肢体肌肉生物能量学，生物力学和灌注研究（Kushmerick, Conley， Marcinek和Marro）将继续使用这种高场仪器进行新的研究，在空间分辨率和信号噪声方面有明显的改进，并且能够利用新的多核和多光谱（光学和核磁共振）方法。大脑中被认为参与喂养和肥胖调节的代谢物的定量（Cummings）将首次在急性实验中进行动态监测，并在长期研究中进行纵向监测。正常小鼠和转基因小鼠（UW的Sze和Miyaoka， FHCRC的Grady， Kemp和Greenberg）的肝脏、脑、前列腺和植入性肿瘤的高分辨率1H和23Na成像将为研究提供方便。对于正在进行的大范围项目来说，这次升级的预算是适度的。