GRADIENT AND RF COIL UPGRADES FOR 47 T IMAGING SPECTROMETER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：肌肉生物学、肥胖症和癌症生物学领域的三个领先研究小组的持续生产力需要在成像梯度、射频线圈和生理选通和监测方面进行重大改进。短径4.7 T Bruker磁铁的问世，促进了一项综合肌肉研究项目的生产力，该项目使用光学和磁共振光谱学来分析人体和动物肌肉的代谢和能量平衡。新的发展将光学近红外光谱与磁共振结合起来研究线粒体功能障碍，并将磁共振灌注方法与代谢通量的光谱测量相结合，以解决影响肌肉的一系列疾病的重要机制。最近与这所大学其他地方的合作者合作的机会使大鼠大脑的光谱成像成为可能，这种成像测量了参与调节摄食和肥胖的细胞机制的分子。我们系和弗雷德·哈钦森癌症研究中心领导的癌症生物学项目将MR和PET的高分辨率小鼠成像和高分辨率小鼠成像结合在一起，这将带来更多的机会。所有这些NIH项目的研究领导都因梯度和射频线圈不足以及缺乏监测磁铁中动物的生理状态和门控的工具而受到威胁。这个应用程序需要资金为这个磁铁购买一个定制的垫片和梯度线圈，一个非常高梯度强度的插入物，用于大鼠和小鼠研究，动物监测设备和射频线圈。我们的仪器所要求的升级将为我们目前资助的12个项目提供所需的灵活性和能力，这些项目位于大学的放射科、医学系和弗雷德·哈钦森癌症研究中心。所有这些项目都因各自领域的卓越而在国际上闻名于世。人体四肢肌肉生物能量学、生物力学和灌注研究(Kushmerick、Conley、Marcinek和Marro)将继续使用这种高场强仪器进行新的研究，在空间分辨率和信噪比以及开发新的多核和多光谱(光学和核磁共振)方法方面有明显的改进。大脑中被认为参与调节摄食和肥胖(Cummings)的代谢物的量化将首次在急性实验期间进行动态监测，并在长期研究中进行纵向监测。肝脏、脑、前列腺和移植肿瘤的高分辨率1H和23Na成像将有助于正常和转基因小鼠的研究(华盛顿大学的Sze和Miyaoka以及FHCRC的Grady、Kemp和Greenberg)。对于正在进行的大范围项目来说，这次升级的预算是适度的。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The continued productivity of three leading research groups in Muscle Biology, Obesity and Cancer Biology require major improvements in imaging gradients, RF coils and physiologic gating and monitoring. The availability of a short bore 4.7 T Bruker magnet catalyzed the productivity of an integrated program of muscle studies using optical and MR spectroscopy to analyze metabolic and energy balances in human and animal muscles. New developments integrate optical NIR spectroscopy with MR in the investigation of mitochondrial dysfunction and integrate MR perfusion methods with spectroscopic measurements of metabolic fluxes to address important mechanisms in a range of maladies affecting muscle. Recent opportunities with collaborators elsewhere at this University enabled spectroscopic imaging of rat brain that measures molecules involved in cellular mechanisms regulating feeding and obesity. Further opportunities exist by combining MR and PET high resolution mouse imaging and in high resolution mouse imaging by leading cancer biology programs in our department and at the Fred Hutchinson Cancer Research Center. Research leadership by all of these NIH projects is jeopardized by inadequate gradients and RF coils and by the absence of tools for monitoring the physiological status of the animals in the magnet and for gating. This application requests funds to purchase a customized shim and gradient coils for this magnet, a very high gradient strength insert for rat and mouse studies, animal monitoring equipment and RF coils. The requested upgrades to our instrument will provide needed flexibility and capabilities for our twelve currently funded projects in the departments of Radiology, and Medicine at the University and at the Fred Hutchinson Cancer Research Center. All of these programs are internationally known for excellence in their fields. Human limb muscle bioenergetics, biomechanics and perfusion studies (Kushmerick, Conley, Marcinek and Marro) will continue their novel studies with this very high field instrument with clear-cut improvements in spatial resolution and signal to noise and in the ability to exploit novel multinuclear and multispectral (optics and NMR) methods. Quantification of metabolites in brain thought to be involved in regulation of feeding and obesity (Cummings) will for the first time be monitored dynamically during acute experiments and longitudinally in long term studies. High resolution 1H and 23Na imaging of liver, brain, prostate and implanted tumors will be facilitated for the studies of normal and transgenic mice (Sze and Miyaoka at UW and Grady, Kemp and Greenberg at FHCRC). The budget for this upgrade is modest for the large scope of projects being done.