GRADIENT AND RF COIL UPGRADES FOR 47 T IMAGING SPECTROMETER

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：肌肉生物学、肥胖症和癌症生物学三个领先研究小组的持续生产力需要在成像梯度、射频线圈以及生理门控和监测方面进行重大改进。短孔 4.7 T 布鲁克磁铁的出现促进了肌肉研究综合计划的生产力，该计划使用光学和 MR 光谱来分析人类和动物肌肉的代谢和能量平衡。新的发展将光学近红外光谱与 MR 结合起来研究线粒体功能障碍，并将 MR 灌注方法与代谢通量的光谱测量相结合，以解决影响肌肉的一系列疾病的重要机制。最近与该大学其他地方的合作者合作，实现了大鼠大脑的光谱成像，测量参与调节进食和肥胖的细胞机制的分子。通过将 MR 和 PET 高分辨率小鼠成像与我们部门和 Fred Hutchinson 癌症研究中心领先的癌症生物学项目相结合的高分辨率小鼠成像，存在进一步的机会。所有这些 NIH 项目的研究领导地位都因梯度和射频线圈不足以及缺乏用于监测磁体中动物的生理状态和门控的工具而受到损害。该申请要求资金购买用于该磁体的定制垫片和梯度线圈、用于大鼠和小鼠研究的非常高梯度强度插入物、动物监测设备和射频线圈。所要求的对我们仪器的升级将为我们目前在大学放射学和医学系以及弗雷德·哈钦森癌症研究中心资助的 12 个项目提供所需的灵活性和功能。所有这些项目都因其在各自领域的卓越表现而享誉国际。人体肢体肌肉生物能量学、生物力学和灌注研究（Kushmerick、Conley、Marcinek 和 Marro）将继续使用这种高场仪器进行新颖的研究，该仪器在空间分辨率和信噪比方面有明显的改进，并且能够开发新颖的多核和多光谱（光学和核磁共振）方法。大脑中被认为与喂养和肥胖调节有关的代谢物（卡明斯）的量化将首次在急性实验中进行动态监测，并在长期研究中进行纵向监测。肝脏、大脑、前列腺和植入肿瘤的高分辨率 1H 和 23Na 成像将有助于正常和转基因小鼠的研究（华盛顿大学的 Sze 和 Miyaoka 以及 FHCRC 的 Grady、Kemp 和 Greenberg）。对于正在进行的大范围项目来说，此次升级的预算并不高。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The continued productivity of three leading research groups in Muscle Biology, Obesity and Cancer Biology require major improvements in imaging gradients, RF coils and physiologic gating and monitoring. The availability of a short bore 4.7 T Bruker magnet catalyzed the productivity of an integrated program of muscle studies using optical and MR spectroscopy to analyze metabolic and energy balances in human and animal muscles. New developments integrate optical NIR spectroscopy with MR in the investigation of mitochondrial dysfunction and integrate MR perfusion methods with spectroscopic measurements of metabolic fluxes to address important mechanisms in a range of maladies affecting muscle. Recent opportunities with collaborators elsewhere at this University enabled spectroscopic imaging of rat brain that measures molecules involved in cellular mechanisms regulating feeding and obesity. Further opportunities exist by combining MR and PET high resolution mouse imaging and in high resolution mouse imaging by leading cancer biology programs in our department and at the Fred Hutchinson Cancer Research Center. Research leadership by all of these NIH projects is jeopardized by inadequate gradients and RF coils and by the absence of tools for monitoring the physiological status of the animals in the magnet and for gating. This application requests funds to purchase a customized shim and gradient coils for this magnet, a very high gradient strength insert for rat and mouse studies, animal monitoring equipment and RF coils. The requested upgrades to our instrument will provide needed flexibility and capabilities for our twelve currently funded projects in the departments of Radiology, and Medicine at the University and at the Fred Hutchinson Cancer Research Center. All of these programs are internationally known for excellence in their fields. Human limb muscle bioenergetics, biomechanics and perfusion studies (Kushmerick, Conley, Marcinek and Marro) will continue their novel studies with this very high field instrument with clear-cut improvements in spatial resolution and signal to noise and in the ability to exploit novel multinuclear and multispectral (optics and NMR) methods. Quantification of metabolites in brain thought to be involved in regulation of feeding and obesity (Cummings) will for the first time be monitored dynamically during acute experiments and longitudinally in long term studies. High resolution 1H and 23Na imaging of liver, brain, prostate and implanted tumors will be facilitated for the studies of normal and transgenic mice (Sze and Miyaoka at UW and Grady, Kemp and Greenberg at FHCRC). The budget for this upgrade is modest for the large scope of projects being done.