MOLECULAR EPIDEMIOLOGY OF OVARIAN CANCER

描述：这是一个基于人群的病例对照应用程序 北卡罗来纳州卵巢癌研究。 它建议进行家庭 对700名新病例进行了采访和抽血， 报告系统和700个控制，通过随机数字识别 拨号（RDD）和卫生保健融资管理局（HCFA）文件。 将检测肿瘤组织的p53突变和HER-2/neu 扩增：先用免疫组化染色补充 通过测序;第二个使用FISH。 基因组DNA将被检测 TP 53、H-Ras、CYP 17和环氧化物水解酶基因的多态性。 一般的假设是卵巢癌是一种异质性疾病 由几种不同的突变途径引起 申请书提出， 为了检验这一点，我们分析了由患有或 没有p53突变，不同组织学类型和不同 多态性 发表的初步研究已经表明， 卵巢肿瘤中p53异常表达的相关性， 排卵的流行病学标志物。

DESCRIPTION: This is an application for a population-based, case-control study of ovarian cancer in North Carolina. It proposes to conduct home interviews and take blood from 700 new cases, identified through a rapid reporting system, and 700 controls, identified through random digit dialing (RDD) and Health Care Financing Administration (HCFA) files. Tumor tissues will be tested for p53 mutations and for HER-2/neu amplification: the first using immunohistochemical staining supplemented by sequencing; and the second using FISH. Genomic DNA will be tested for polymorphisms in the genes for TP53, H-Ras, CYP17 and epoxide hydrolase. The general hypothesis is that ovarian cancer is a heterogeneous disease caused by several different mutational pathways. The application proposes to test this by analyzing subgroups of the data defined by tumors with or without mutations in p53, different histologic types and different polymorphisms. Published preliminary studies have already shown an association with abnormal expression of p53 in ovarian tumors and epidemiologic markers of ovulation.