MOLECULAR EPIDEMIOLOGY OF OVARIAN CANCER

描述：这是一个以人口为基础的病例对照的应用程序 北卡罗来纳州卵巢癌的研究。它建议将家庭 约谈并从700例新病例中抽血，通过快速 报告系统和700个控件，通过随机数字标识 拨号(RDD)和医疗保健融资管理(HCFA)文件。 肿瘤组织将进行P53突变和HER-2/neu检测 扩增：首次采用免疫组织化学染色补充 通过测序；第二种使用鱼类。将对基因组DNA进行检测 TP53、H-Ras、CyP17和环氧化物水解酶基因的多态性 一般的假设是卵巢癌是一种异质性疾病。 由几种不同的突变途径引起。该申请提出了 通过分析由具有或的肿瘤定义的数据的子组来测试这一点 不同的组织学类型和不同的P53突变 多态现象。已发表的初步研究已经表明， 卵巢肿瘤组织中P53蛋白异常表达与预后的关系 排卵的流行病学标志物。

DESCRIPTION: This is an application for a population-based, case-control study of ovarian cancer in North Carolina. It proposes to conduct home interviews and take blood from 700 new cases, identified through a rapid reporting system, and 700 controls, identified through random digit dialing (RDD) and Health Care Financing Administration (HCFA) files. Tumor tissues will be tested for p53 mutations and for HER-2/neu amplification: the first using immunohistochemical staining supplemented by sequencing; and the second using FISH. Genomic DNA will be tested for polymorphisms in the genes for TP53, H-Ras, CYP17 and epoxide hydrolase. The general hypothesis is that ovarian cancer is a heterogeneous disease caused by several different mutational pathways. The application proposes to test this by analyzing subgroups of the data defined by tumors with or without mutations in p53, different histologic types and different polymorphisms. Published preliminary studies have already shown an association with abnormal expression of p53 in ovarian tumors and epidemiologic markers of ovulation.