MAMMAGLOBIN GENE IN BREAST DEVELOPMENT AND NEOPLASIA

描述：乳大球蛋白是一种最近分离的新蛋白质，它是 与一个分泌型配体结合蛋白家族同源，发现于 上皮组织。MAMMALOBIN的表达严格限于 乳腺。此外，乳大球蛋白的mRNA和蛋白表达上调。 在相当大比例的乳腺癌细胞系和组织中。在……里面 这项建议提出了三个目的：一是基本转录 调控元素将通过比较启动子缺失和 乳腺和非乳腺上皮细胞株的凝胶迁移率变化分析； 第二，表位标记和抗巨球蛋白抗血清的制备 用于检查四元结构、翻译调节和 潜在的配基结合能力；最后，启动子的功能 第一个目标中确定的元素将在转基因生物中进行测试 鼠标模型系统。

DESCRIPTION: Mammaglobin is a recently isolated, novel protein that is homologous to a family of secreted, ligand-binding proteins found in epithelial tissues. Mammaglobin expression is strictly limited to the mammary gland. Furthermore, mammaglobin mRNA and protein are up-regulated in a substantial proportion of breast carcinoma cell lines and tissues. In this proposal three aims are proposed: first, the basic transcriptional regulatory elements will be identified by comparative promoter deletion and gel mobility shift assays in breast and non-breast epithelial cell lines; second, epitope tagging and creating of anti-mammaglobin antisera will be used to examine the quaternary structure, translational regulation, and potential ligand binding capacity; last, the functionality of the promoter elements identified in the first objective will be tested in a transgenic mouse model system.