MAMMAGLOBIN GENE IN BREAST DEVELOPMENT AND NEOPLASIA

乳腺珠蛋白基因与乳房发育和肿瘤的关系

• 批准号: 6489118
• 负责人: Mark A. Watson
• 金额: $ 11.38万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6489118
• 关键词: DNA footprinting; HeLa cells; breast neoplasms; cell line; epitope mapping; gene deletion mutation; gene expression; genetic promoter element; genetically modified animals; globin; hormone binding protein; laboratory mouse; mammary epithelium; nucleic acid sequence; polymerase chain reaction; posttranslational modifications; site directed mutagenesis; transcription factor

DESCRIPTION: Mammaglobin is a recently isolated, novel protein that is homologous to a family of secreted, ligand-binding proteins found in epithelial tissues. Mammaglobin expression is strictly limited to the mammary gland. Furthermore, mammaglobin mRNA and protein are up-regulated in a substantial proportion of breast carcinoma cell lines and tissues. In this proposal three aims are proposed: first, the basic transcriptional regulatory elements will be identified by comparative promoter deletion and gel mobility shift assays in breast and non-breast epithelial cell lines; second, epitope tagging and creating of anti-mammaglobin antisera will be used to examine the quaternary structure, translational regulation, and potential ligand binding capacity; last, the functionality of the promoter elements identified in the first objective will be tested in a transgenic mouse model system.

描述：乳腺珠蛋白是一种最近分离的新型蛋白质， 与一个分泌的配体结合蛋白家族同源， 上皮组织 乳腺珠蛋白的表达严格限制在 乳腺 此外，乳腺珠蛋白mRNA和蛋白质表达上调， 在相当大比例的乳腺癌细胞系和组织中。 在 该提案提出了三个目标：第一，基本转录 调节元件将通过比较启动子缺失来鉴定， 在乳腺和非乳腺上皮细胞系中的凝胶迁移率变化测定; 第二，表位标记和抗乳房珠蛋白抗血清的产生将是 用于检查四级结构，翻译调节， 潜在的配体结合能力;最后，启动子的功能 在第一个目标中鉴定的元件将在转基因植物中进行测试。 小鼠模型系统。