Core--Multiplexed Gene Analysis

多路基因分析(MGA)核心目前为癌症中心成员提供高通量基因表达分析技术。这些技术往往与相当大的基础设施、成本、质量控制和/或复杂性有关，因此很难在个别实验室建立。MGA Core使用Affymetrix基因芯片？寡核苷酸微阵列提供基因表达分析服务。服务包括阵列杂交和从标准(3-5微克)和小规模(10-30毫微克)RNA样本制备标记靶标。阵列数据直接集成到SCC生物信息学核心中，在那里可以使用数据检索和数据挖掘工具。特别强调数据质量保证和样本跟踪。MGA Core还提供定量(实时)RT-PCR服务。调查人员可以选择准备他们自己的分析，然后提交给核心进行分析。或者，使用商业上可买到的、预先验证的引物集， CORE将使用研究人员提供的RNA执行基因表达分析的所有步骤。未来，基于阵列和基于聚合酶链式反应的SNP检测将在Core的现有技术平台上得到支持。在过去的一年里，Core已经为40多个癌症中心成员项目生成了基因表达数据，因此继续成为基础和转化性癌症研究的重要技术资源。

The Multiplexed Gene Analysis (MGA) Core currently provides Cancer Center members with access to technologies for high-throughput gene expression analysis. These technologies often are associated with considerable infrastructure, cost, quality control and/or complexity, making them difficult to establish in individual laboratories. The MGA Core provides gene expression profiling services using Affymetrix GeneChip ?oligonucleotide microarrays. Services include array hybridization and labeled target preparation from standard (3-5 ug) and small scale (10-30 ng) RNA samples. Array data is directly integrated into the SCC Bioinformatics Core, where data retrieval and data mining tools are made available. Particular emphasis is placed on data quality assurance and sample tracking. The MGA Core also provides quantitative (real-time) RT-PCR services. Investigators may elect to prepare their own assays that are then submitted to the Core for analysis. Alternatively, using commercially available, pre-validated primer sets, the Core will perform all steps of the gene expression assay with RNA provided by the investigator. In the future, array-based and PCR-based SNP assays will be supported on the Core's existing technology platforms. Within the past year, the Core has generated gene expression data for over forty cancer center member projects, thus continuing to be a significant technical resource for both basic and translational cancer research.