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AUTORADIOGRAPHY & PHYSIOLOGY OF GASTROINTESTINE PEPTIDES

放射自显影

基本信息

批准号：
3072448
负责人：
Ann Ouyang
金额：
$ 5.6万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-09-01 至 1991-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3072448
关键词：
adrenergic receptor autoradiography bombesin cats cholinergic receptors endorphins enkephalins esophagogastric junction gastrointestinal hormones gastrointestinal motility /pressure neuropeptide receptor neuropeptides neurophysiology neurotransmitters opiate alkaloid physiology pyloric region substance P

项目摘要

The research plan consists of two major projects. The first is to define the physiologic responses of the feline ileocecal sphincter (ICS) to feeding and to local stimuli such as distension, and to determine the contribution of various components of the anatomic ICS to these physiologic responses. These studies will contribute to an understanding of the integrative role, if any, of the ICS to the differing functions of the ileum and colon. The project consists of using three methods, an in vivo method and two in vitro methods to examine these problems. Discrepancy exists in the literature between studies performed in vivo and in vitro. The proposed studies will be directed to determine the reason for such discrepancies. The pathways mediating ICS relaxation to proximal distension, and contraction to distal distension will be defined. The effect of many peptides which are either released after meals or have been described to be present in the ICS, and their sites of action will be examined in vivo and in vitro. The integrative function of the ileum, ICS and colon in this area will be examined using myotomy techniques in vivo and in vitro. In addition, the possible role of vasoactive intestinal peptide as the neurotransmitter mediating relaxation at the ICS will be studied. The second major project of this research plan is the development of the technique of autoradiography of peptide receptors in the gastrointestinal tract. The methodology for autoradiography of opiate-, substance P-, bombesin-, as well as cholinergic and adrenergic receptors will be determined. This technique has not previously been applied to the gastrointestinal tract with respect to neuropeptides and neurotransmitters relevant to gastrointestinal motility. Quantitative autoradiography will allow the comparison of receptor sites and densities in different areas of the gastrointestinal tract and allow a comparison of the receptor population with physiologic responses in vivo and in vitro.

该研究计划包括两个主要项目。一是定义猫回盲括约肌的生理反应 (ICS)进食和局部刺激，如膨胀，以及确定解剖结构的各个组成部分的贡献 ICS对这些生理反应。这些研究将有助于理解综合服务中心的综合作用，回肠和结肠的不同功能。项目包括使用三种方法，一种体内方法和两种体内方法，体外方法来检查这些问题。不确定性存在于体内和体外研究之间的文献。的建议的研究将直接确定这种情况的原因，差异介导ICS松弛的途径，近端扩张和收缩到远端扩张将是定义了许多肽的作用，这些肽要么被释放，餐后或已被描述为存在于ICS中，以及将在体内和体外研究它们的作用部位。的回肠、ICS和结肠在该区域的整合功能将在体内和体外使用肌切开术技术进行检查。在此外，血管活性肠肽可能作为神经递质介导的ICS放松将被研究。这项研究计划的第二个主要项目是开发肽受体的放射自显影技术，胃肠道放射自显影的方法学阿片类药物，P物质，蛙皮素，以及胆碱能和将测定肾上腺素能受体。这项技术没有先前应用于胃肠道，神经肽和神经递质，胃肠蠕动。定量放射自显影术将允许不同地区受体位点和密度的比较的胃肠道，并允许比较受体群体与体内和体内的生理反应体外