POSTNATAL DEVELOPMENT OF EXPIRATORY MUSCLE

这位候选人是麻省理工学院儿科三年级助理教授 匹兹堡大学医学院，并致力于 学术医学证明了他的研究努力和对 教书。他一直对呼吸肌在其中扮演的角色感兴趣。 新生儿呼吸衰竭的发生发展。他正计划着 通过在以下方面获得新技能来扩大他研究的广度 肌肉生物化学和体外肌肉收缩生理学 本申请中提出的培训计划。这些新技能是 确保未来成功进行独立调查所必需的 呼吸肌发育。 拟议调查的主要研究目标是确定 出生后收缩、生化和耐力特性的变化 指腹外斜肌(呼气性腹肌 “混合”快速和慢速抽动纤维种群(成熟时)。生化 要进行的评估包括对这块肌肉的肌球蛋白进行分析 收缩蛋白组成，其纤维琥珀酸脱氢酶(SDH) 活性及其肌原纤维ATPase活性。我们计划将其与 肌原纤维ATPase和SDH活性的耐力特性。 对肋隔膜(AN)进行相同的评估 “混合型”快慢抽动肌纤维的吸气肌控制 成熟时的人口)和腓肠肌内侧(附件 “混合”快慢抽动纤维群体的肌肉控制 成熟)，以吸引呼气-吸气，呼吸-阑尾， 以及吻尾比较。通过描述这些发展中的 在呼气和吸气肌肉中的特性，我们将加深我们的 对开发中的呼吸机功能潜力的理解 打气筒。

The candidate is a third year Assistant Professor of pediatrics at the University of Pittsburgh School of Medicine and has a strong commitment to academic medicine as evidenced by his research endeavors and commitment to teaching. He has been interested in the role respiratory muscles play in the development of ventilatory failure in newborns. He is planning to expand the breadth of his research studies by acquiring new skills in muscle biochemistry and in vitro muscle contractile physiology during the training program proposed in the present application. These new skills are necessary to ensure successful future independent investigators of respiratory muscle development. The primary research goal of the proposed investigation is to determine the postnatal changes in the contractile, biochemical, and endurance properties of the external abdominal oblique muscle (an expiratory abdominal muscle of "mixed" fast and slow twitch fiber population when mature). Biochemical assessments to be performed include an analysis of this muscle's myosin contractile protein composition, its fiber succinate dehydrogenase (SDH) activity and its myofibrillar ATPase activity. We plan to correlate endurance properties with myofibrillar ATPase and SDH activities. Identical assessments will be performed on the costal diaphragm (an inspiratory muscle control of "mixed" fast and slow twitch muscle fiber population when mature), and the medial gastrocnemius (an appendicular muscle control of "mixed" fast and slow twitch fiber population when mature) in order to draw expiratory-inspiratory, ventilatory-appendicular, and rostral-caudal comparisons. By characterizing these developmental properties in expiratory and inspiratory muscle, we will deepen our understanding of the functional potential of the developing ventilatory pump.