MOLECULAR BIOLOGY OF THYMULIN

胸腺素的分子生物学

• 批准号: 3816718
• 负责人: JUN HAYASHI HAYASHI
• 金额: --
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3816718
• 关键词: bioassay; complementary DNA; enzyme linked immunosorbent assay; epithelium; gene expression; genetic library; hormone regulation /control mechanism; laboratory mouse; laboratory rat; leukopoiesis; molecular cloning; nucleic acid probes; peptide hormone; peptide hormone biosynthesis; protein purification; thymus; tissue /cell culture

Nonapeptide thymic hormone thymulin is produced by thymic endocrine epithelial cells. Its biological activities have been well described and its role in various immunopathological status including AIDS has been implicated. Although its complete amino acid sequence has been established more than 10 years ago, the presence of prothymulin was not discovered till recently. Moreover, its gene has not been cloned yet. The advancement in the field of molecular biology of thymulin has been severely hindered by the lack of in vitro model system. Here we report the recent establishment of thymic endocrine epithelial cell line which produces thymulin and proposes to: 1) purify and sequence prothymulin; 2) clone thymulin gene and 3) study the control mechanism of thymulin gene activation and expression. We are now developing cDNA library from thymic endocrine epithetial cell line. We have also developed rabbit antibody to thymulin which recognizes prothymulin molecule as well. With this cell line and antiserum in our hand, we are confident that we can achieve the proposed study.

非肽胸腺激素胸腺素是由胸腺内分泌产生的 上皮细胞。它的生物活性已经得到了很好的描述和 它在包括艾滋病在内的各种免疫病理状态中的作用一直是 牵连其中。尽管其完整的氨基酸序列已经确定 10多年前，原百里香素的存在才被发现 最近。此外，它的基因还没有被克隆。在中国的进步 百里香的分子生物学领域受到了严重的阻碍 缺乏体外模型系统。在这里，我们报道最近成立的 胸腺内分泌上皮细胞株产生胸腺激素和 提出：1)纯化和测序原百里香；2)克隆百里香基因 3)研究胸腺细胞因子基因激活的调控机制。 表情。我们正在开发胸腺内分泌的cdna文库。 上皮细胞系。我们还研制出了针对百里香素的兔抗体 它也能识别原百里香分子。在这个细胞系中 在我们手中有抗血清的情况下，我们有信心实现拟议的 学习。