THYMIC STROMAL CELLS AND T CELL DIFFERENTIATION

胸腺基质细胞和 T 细胞分化

• 批准号: 3897532
• 负责人: JUN HAYASHI HAYASHI
• 金额: --
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3897532
• 关键词: DNA directed DNA polymerase; T lymphocyte; arachidonate; bone marrow; cell differentiation; cell growth regulation; deficient growth media; hematopoietic stem cells; hormone regulation /control mechanism; immunofluorescence technique; laboratory mouse; laboratory rat; lymphoblast; phospholipase A2; radioimmunoassay; stromal cells; thymus; tissue /cell culture

It has been postulated by various investigators that cells which possess enzyme terminal deoxynucleotidyl transferase in the bone marrow of young adult experimental mammals and birds are T cell progenitors. Recently, an in vitro culture method which promotes the growth of rat bone marrow TdT-positive T cell progenitor cells has been established. This culture system requires a continuous presence of an adherent feeder layer from mouse bone marrow cells. TdT-positive cells from rat bone marrow selectively proliferate in the suspension phase of the culture media when a healthy mouse bone marrow feeder layer is present. We have also established a culture method for growing thymic epithelial cells in serum-free culture conditions. From these primary culture, a cell line (TEA3A1 cells) was established. TEA3A1 cells produce biological activity which stimulates the (3H)-TdR incorporation of ConA-stimulated thymocytes. This activity is different from I1-2. In this application we propose to examine the developmental potential of bone marrow TdT-positive cells and the functional capacity of various thymic stromal cells including TEA3A1 cells to support the growth and/or differentiation of TdT-positive cells.

不同的研究人员假设细胞 骨中具有末端脱氧核苷酸转移酶 年轻成年实验哺乳动物和鸟类的骨髓是 T 细胞 祖先。 最近，一种体外培养方法可促进 大鼠骨髓TdT阳性T细胞祖细胞的生长 细胞已经建立。 这个文化体系需要 小鼠骨中连续存在粘附的饲养层 骨髓细胞。 来自大鼠骨髓的 TdT 阳性细胞 在培养物的悬浮阶段选择性增殖 当存在健康小鼠骨髓饲养层时的培养基。 我们还建立了胸腺生长的培养方法 无血清培养条件下的上皮细胞。 从这些 原代培养，建立细胞系（TEA3A1细胞）。 TEA3A1 细胞产生生物活性，刺激 (3H)-TdR 掺入 ConA 刺激的胸腺细胞。 这 活性与 I1-2 不同。 在此应用中，我们建议检查发育 骨髓TdT阳性细胞的潜力及其功能 包括TEA3A1细胞在内的各种胸腺基质细胞的能力 支持 TdT 阳性细胞的生长和/或分化。