MOLECULAR BIOLOGY OF THYMULIN

胸腺素的分子生物学

• 批准号: 3794985
• 负责人: JUN HAYASHI HAYASHI
• 金额: --
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3794985
• 关键词: bioassay; complementary DNA; enzyme linked immunosorbent assay; epithelium; gene expression; gene induction /repression; genetic library; hormone regulation /control mechanism; laboratory mouse; laboratory rat; leukopoiesis; molecular cloning; nucleic acid probes; peptide hormone; peptide hormone biosynthesis; protein purification; thymus; tissue /cell culture

Nonapeptide thymic hormone thymulin is produced by thymic endocrine epithelial cells. Its biological activities have been well described and its role in various immunopathological status including AIDS has been implicated. Although its complete amino acid sequence has been established more than 10 years ago, the presence of prothymulin was not discovered till recently. Moreover, its gene has not been cloned yet. The advancement in the field of molecular biology of thymulin has been severely hindered by the lack of in vitro model system. Here we report the recent establishment of thymic endocrine epithelial cell line which produces thymulin and proposes to: 1) purify and sequence prothymulin; 2) clone thymulin gene and 3) study the control mechanism of thymulin gene activation and expression. We are now developing cDNA library from thymic endocrine epithetial cell line. We have also developed rabbit antibody to thymulin which recognizes prothymulin molecule as well. With this cell line and antiserum in our hand, we are confident that we can achieve the proposed study.

九肽胸腺激素胸腺肽由胸腺内分泌产生 上皮细胞 它的生物活性已经得到很好的描述， 它在包括AIDS在内各种免疫病理状态中的作用， 牵连 虽然其完整的氨基酸序列已经确定 10多年前，人们才发现胸腺素原的存在， 最近 此外，它的基因还没有被克隆。 的进步 胸腺肽的分子生物学领域受到严重阻碍， 缺乏体外模型体系。 在这里，我们报告最近建立的 胸腺内分泌上皮细胞系产生胸腺肽， 拟：1）纯化胸腺肽原并测序; 2）克隆胸腺肽基因 研究胸腺肽基因激活的调控机制， 表情 我们正在从胸腺内分泌细胞中构建cDNA文库， 上皮细胞系 我们还研制了兔抗胸腺肽抗体 它也识别胸腺肽原分子。 用这个细胞系， 在我们手中的抗血清，我们有信心，我们可以实现拟议的 study.