THYMIC STROMAL CELLS AND T CELL DIFFERENTIATION

胸腺基质细胞和 T 细胞分化

• 批准号: 3876115
• 负责人: JUN HAYASHI HAYASHI
• 金额: --
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3876115
• 关键词: DNA directed DNA polymerase; T lymphocyte; arachidonate; bone marrow; cell differentiation; cell growth regulation; deficient growth media; hematopoietic stem cells; hormone regulation /control mechanism; immunofluorescence technique; laboratory mouse; laboratory rat; lymphoblast; phospholipase A2; radioimmunoassay; stromal cells; thymus; tissue /cell culture

It has been postulated by various investigators that cells which possess enzyme terminal deoxynucleotidyl transferase in the bone marrow of young adult experimental mammals and birds are T cell progenitors. Recently, an in vitro culture method which promotes the growth of rat bone marrow TdT-positive T cell progenitor cells has been established. This culture system requires a continuous presence of an adherent feeder layer from mouse bone marrow cells. TdT-positive cells from rat bone marrow selectively proliferate in the suspension phase of the culture media when a healthy mouse bone marrow feeder layer is present. We have also established a culture method for growing thymic epithelial cells in serum-free culture conditions. From these primary culture, a cell line (TEA3A1 cells) was established. TEA3A1 cells produce biological activity which stimulates the (3H)-TdR incorporation of ConA-stimulated thymocytes. This activity is different from I1-2. In this application we propose to examine the developmental potential of bone marrow TdT-positive cells and the functional capacity of various thymic stromal cells including TEA3A1 cells to support the growth and/or differentiation of TdT-positive cells.

不同的研究人员推测，细胞 骨骼中具有酶末端脱氧核苷酸转移酶 幼年成年实验哺乳动物和鸟类的骨髓是T细胞 祖先。最近，一种体外培养方法促进了 大鼠骨髓TDT阳性T细胞前体细胞的生长 细胞已经建立。这个文化系统需要一个 连续存在的小鼠骨粘附性饲养层 骨髓细胞。大鼠骨髓中的TDT阳性细胞 在培养的悬浮阶段选择性地增殖 当存在健康的小鼠骨髓饲养层时，进行培养。 我们还建立了一种培养胸腺的方法 无血清培养条件下的上皮细胞。从这些 通过原代培养，建立了TEA3A1细胞系。 TEA3A1细胞产生生物活性，刺激 ConA刺激的胸腺细胞的(~3H)-TdR掺入。这 活动不同于I1-2。 在本应用程序中，我们建议检查开发的 骨髓TDT阳性细胞的潜能及其功能 包括TEA3A1细胞在内的各种胸腺基质细胞的能力 以支持TDT阳性细胞的生长和/或分化。