THYMIC STROMAL CELLS AND T CELL DIFFERENTIATION

胸腺基质细胞和 T 细胞分化

• 批准号: 3918069
• 负责人: JUN HAYASHI HAYASHI
• 金额: --
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3918069
• 关键词: DNA directed DNA polymerase; T lymphocyte; arachidonate; bone marrow; cell differentiation; cell growth regulation; deficient growth media; hematopoietic stem cells; hormone regulation /control mechanism; immunofluorescence technique; laboratory mouse; laboratory rat; lymphoblast; phospholipase A2; radioimmunoassay; stromal cells; thymus; tissue /cell culture

It has been postulated by various investigators that cells which possess enzyme terminal deoxynucleotidyl transferase in the bone marrow of young adult experimental mammals and birds are T cell progenitors. Recently, an in vitro culture method which promotes the growth of rat bone marrow TdT-positive T cell progenitor cells has been established. This culture system requires a continuous presence of an adherent feeder layer from mouse bone marrow cells. TdT-positive cells from rat bone marrow selectively proliferate in the suspension phase of the culture media when a healthy mouse bone marrow feeder layer is present. We have also established a culture method for growing thymic epithelial cells in serum-free culture conditions. From these primary culture, a cell line (TEA3A1 cells) was established. TEA3A1 cells produce biological activity which stimulates the (3H)-TdR incorporation of ConA-stimulated thymocytes. This activity is different from I1-2. In this application we propose to examine the developmental potential of bone marrow TdT-positive cells and the functional capacity of various thymic stromal cells including TEA3A1 cells to support the growth and/or differentiation of TdT-positive cells.

许多研究者都假设， 在骨中具有酶末端脱氧核苷酸转移酶 成年实验哺乳动物和鸟类的骨髓是T细胞 祖先 最近，一种体外培养方法， 大鼠骨髓TdT阳性T细胞祖细胞生长 细胞已经建立。 这种文化需要一个 持续存在来自小鼠骨的粘附性饲养层 骨髓细胞 大鼠骨髓TdT阳性细胞 选择性地在培养物的悬浮阶段增殖 当存在健康小鼠骨髓饲养层时， 我们还建立了胸腺细胞的培养方法， 上皮细胞在无血清培养条件下。 从这些 在原代培养中，建立细胞系（TEA 3A 1细胞）。 TEA 3A 1细胞产生生物活性，其刺激 ConA刺激的胸腺细胞的（3 H）-TdR掺入。 这 活性与I1-2不同。 在本申请中，我们建议检查 骨髓TdT阳性细胞的潜能和功能 各种胸腺基质细胞包括TEA 3A 1细胞的能力 以支持TdT阳性细胞的生长和/或分化。