REGULATION AND EVOLUTION OF MULTIGENE FAMILIES

多基因家族的调控和进化

• 批准号: 3272126
• 负责人: FOTIS C KAFATOS
• 金额: $ 22.01万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3272126
• 关键词: DNA; animal population genetics; chorioallantoic membrane; developmental genetics; eukaryote; evolution; gene conversion; gene expression; genetic manipulation; molecular cloning; mutant; nucleic acid sequence; structural genes

In recent years, it has been firmly established that multigene families are a ubiquitous and important feature in the genomes of higher eucaryotes. Gene families are especially important since they often encode complex aspects of differentiated cell phenotypes and may play key roles in developmental and physiological regulation. Gene families are also important because they reveal the processes that both diversify eucaryotic genomes and maintain certain genetic features relatively invariant--including sequence elements that may be involved in developmental regulation. We are studying that evolution of structural gene families and of the regulatory elements controlling their developmental expression, using as a model system the silkmoth chorion multigene families. We want to document how the structural genes in these families have evolved, by localized fixation of mutations (subtitutions and deletions/duplications) as well as by gene conversion. Furthermore, we want to compare and contrast the evolution of the structural chorion genes with the evolution of non-transcribed sequences that may be involved in the regulation of gene expression. The fundamental approach here is to define precisely families, and to relate it to the sequence features of these genes and their immediately flanking DNA. Finally, we wish to pursue our analysis of the morphogenesis of the complex chorion structure, by taking advantage of variations established during evolution as well as studying some newly discovered chorion mutants in Bombyx mori.

近年来，多基因家族已被证实 高等真核生物基因组中普遍存在的重要特征。 基因家族尤其重要，因为它们通常编码复杂的 分化细胞表型的各个方面，并可能在 发育和生理调节。 基因家族也 重要的是因为它们揭示了真核生物多样化的过程 基因组并相对保持某些遗传特征 不变的——包括可能涉及的序列元素 发育调节。 我们正在研究结构基因家族的进化和 控制其发育表达的调控元件，用作 蚕绒毛膜多基因家族模型系统。 我们想要记录 这些家族的结构基因是如何通过局部进化而进化的 突变的固定（替换和删除/重复）以及 通过基因转换。 此外，我们想要比较和对比 绒毛膜结构基因的进化 可能参与基因调控的非转录序列 表达。 这里的基本方法是精确定义家庭， 并将其与这些基因的序列特征及其相关性联系起来 紧邻DNA的侧翼。 最后，我们希望继续分析 复杂绒毛膜结构的形态发生，通过利用 进化过程中建立的变异以及研究一些新的 在家蚕中发现了绒毛膜突变体。