NATIONAL TRITIUM LABELING FACILITY 20.

国家氚标记设施 20。

• 批准号: 3103801
• 负责人: HENRY RAPOPORT
• 金额: $ 83.25万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-08-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3103801
• 关键词: biomedical resource; chemical synthesis; radiopharmacology; radiotracer

The National Tritium Labeling Facility (NTLF) was funded on 08/01/82. During its first year the facility was built and equipped; it became fully operative in August 1983. It is now engaged in an active program of aiding biomedical researchers to obtain needed and commercially-unavailable tritium labeled compounds. For the ten month period Oct. 1983-Aug. 1984 this has averaged two labeling operations per month and has aided scientists from as far away as New York and Hawaii. The compounds labeled have included prednisone, nicotine, pheromones, gibberelic acids, endorphins, tabtoxinine- -lactam, and tuftsin. The tritium facility is now fully staffed, and because knowledge of its labeling services is growing, requests for tritiation help are also growing; the facility is expected to reach its full capacity (one tritiation per week) in its 3rd year. The core research is being rapidly pursued. During the 2nd grant year one manuscript was accepted for publication in the Journal of Physical Chemistry, and a second manuscript was submitted to that journal in September 1984. The core research summarized in these manuscripts contributes to our understanding of the processes involved in "excitation labeling" (exposure of organic compounds to a stream of T2 that has been passed through a microwave generator). The knowledge gained in this work has increased the efficiency with which the excitation procedure can be used. This research will be enhanced in the 3rd and succeeding grant years by the tritium-NMR capability that has been added. The position(s) of tritium in all labeled compounds can now be determined, and this greatly aids the mechanistic interpretations regarding the tritiation procedures that have been used. The staff of the NTLF is doing collaborative research (co-authorship of published papers) with Professor Richard E. Moore of the University of Hawaii; the work is directed towards identifying the cell-membrane receptor sites for the potent tumor promoters lyngbyatoxin and aplysiatoxin. The staff is also collaborating with Professor A.J. Kresge of the University of Toronto on hydrogen-isotope effects on NMR spectra. This kind of collaborative research will be expanded with the increasing use of the NTLF by biomedical researchers.

国家氚标记机构(NTLF)于1982年1月8日获得资助。 在第一年，该设施建成并配备了设备；它完全 1983年8月投入使用。它现在正在进行一项积极的援助计划 生物医学研究人员将获得所需的和商业上无法获得的 氚标记化合物。1983年10月至8月的10个月期间1984年 这平均每月进行两次贴标签操作，并有助于 远在纽约和夏威夷的科学家。标记的化合物 包括泼尼松、尼古丁、信息素、赤霉酸， 内啡肽、烟碱--内酰胺和Tuftsin。氚设施现在是 人员配备齐全，而且由于对其标签服务的了解正在增长， 对氚帮助的请求也在增加；该设施预计将 在第三年达到满负荷(每周一次)。 核心研究正在迅速展开。在第一年的第二次资助期间 稿件被《物理杂志》接受发表 化学，并于#年向该杂志提交了第二份手稿 1984年9月。这些手稿中总结的核心研究 有助于我们对“激发”过程的理解 标记“(将有机化合物暴露在T2流中 通过微波发生器)。在这项工作中获得的知识 提高了激励程序的效率 使用。这项研究将在第三年和以后的资助年度得到加强。 通过添加的氚-核磁共振能力。职务(S) 现在可以测定所有标记化合物中的氚，这大大 有助于对审判程序进行机械性的解释 已经被使用过的。 国家自然基金会的工作人员正在进行合作研究( 发表的论文)与加州大学的理查德·E·摩尔教授 夏威夷；这项工作的目的是识别细胞膜受体 有效的肿瘤促进剂山雀毒素和海龙毒素的位置。这个 工作人员还与加州大学的A.J.Kresge教授合作 多伦多关于氢同位素对核磁共振谱的影响。这样一种 随着国家技术合作框架的使用增加，合作研究将得到扩大 由生物医学研究人员提供。