Mass spectrometry of protein complexes - from networks to structures

蛋白质复合物的质谱分析 - 从网络到结构

• 批准号: BB/F021208/2
• 负责人: Carol Robinson
• 金额: $ 32.72万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF021208%2F2
• 关键词: Mass; spectrometry; protein; complexes; networks

Mass spectrometry is well established in the fields of proteomics and metabolomics. Much less established is its role in deciphering the overall architecture of protein complexes. Using a procedure whereby we begin with the intact assembly we have shown that we can decompose the assembly into its simplest building blocks, look at their overall shape and then integrate them into 3D models of the overall complex. In order to do this we first have to validate the method using complexes of known structure. We have collaborated with a number of groups worldwide to obtain complexes with a variety of different topological arrangements and structures. Through a systematic survey of these complexes we hope to determine whether or not this approach will be suitable for use in deciding the overall architecture of unknown protein complexes. A further stream of our research involves isolating complexes at endogenous expression levels using a modified isolation protocol in which a single subunit is tagged and complexes are purified using affinity methods. We propose to continue and consolidate very exciting preliminary data that we have obtained for complexes involved in translation and splicing of RNA. The first of this series of complexes consists of the elongation initiation factors. By comparing and contrasting both human and yeast complexes we hope to gain novel insight into their subunit interaction maps and overall architecture. Similarly for the complexes involved in splicing, both yeast and human complexes will be studied, either in collaboration with Olga and E Makarov or by isolation from yeast strains grown in-house.

质谱法在蛋白质组学和代谢组学领域中已得到很好的应用。它在破译蛋白质复合物的整体结构中的作用要少得多。使用一个程序，我们开始与完整的组件，我们已经表明，我们可以分解成最简单的组件，看看他们的整体形状，然后将它们集成到三维模型的整体复杂。为了做到这一点，我们首先必须使用已知结构的复合物来验证该方法。我们已经与世界各地的一些团体合作，以获得具有各种不同拓扑排列和结构的配合物。通过对这些复合物的系统调查，我们希望确定这种方法是否适用于决定未知蛋白质复合物的整体结构。我们的研究的进一步流涉及使用修改的分离方案在内源性表达水平分离复合物，其中单个亚基被标记，并且复合物使用亲和方法纯化。我们建议继续和巩固非常令人兴奋的初步数据，我们已经获得的复合物参与翻译和剪接的RNA。这一系列复合物中的第一个由延伸起始因子组成。通过比较和对比人类和酵母复合物，我们希望获得新的见解，他们的亚基相互作用的地图和整体架构。类似地，对于参与剪接的复合物，将与Olga和E Makarov合作或通过从内部生长的酵母菌株中分离来研究酵母和人类复合物。