A spinning disk confocal microscope for live cell imaging in plant animal and fungal cells.

用于植物、动物和真菌细胞活细胞成像的转盘共聚焦显微镜。

• 批准号: BB/F010788/1
• 负责人: P Hussey
• 金额: $ 29.43万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF010788%2F1
• 关键词: spinning; disk; confocal; microscope; live

Robert Hooke in 1665 was the first to introduce the term 'cell' when he was viewing the 'boxes' he saw in slices of cork using one of the earliest optical compound microscopes (two lenses: an objective lens and an eye piece) that he developed. He probably didn't quite realise the significance of this discovery, as it was only when it became apparent that the great majority of organisms are composed of cells that Cell Theory was born. Cell Theory, first proposed by M.J. Schleiden and Theodore Schwann in 1839, states that cells are of universal occurrence and are the basic units of an organism. This theory in still undisputed, although in those days rivals tried. Over 300 years of microscope improvements have led to fascinating discoveries of how cells function. Electron microscopes use beams of electrons rather than light and can magnify by hundreds of thousands of times. However the material being examined although fixed in time is dead. Light microscopes have evolved with the development of laser technology, imaging solutions, computer hardware/software and the use of fluorescent biological/chemical probes. One of the greatest developments in the last few decades has been the confocal laser scanning microscope, which allows the user to generate 3D images of fixed material and short live cell movies in one plane. With the development of confocal microscopy have come parallel developments/improvements. One such development is the spinning disk technology coupled with laser illumination, and high resolution imaging allowing the user to visualise fluorescent probes in cells in 3D with time (4D) i.e in living cells. To understand the role and function of particular constituents of a cell these features are a desirable advantage to a scientist.

1665年，罗伯特·胡克首次提出了“细胞”一词，当时他用自己研发的最早的光学复合显微镜(两个透镜：一个物镜和一个目镜)来观察软木切片中的“盒子”。他可能并没有完全意识到这一发现的意义，因为直到很明显，绝大多数有机体都是由细胞组成的时候，细胞理论才诞生。细胞理论最早由M.J.Schleiden和Theodore Schwann于1839年提出，该理论认为细胞是普遍存在的，是生物体的基本单位。这一理论仍然是无可争议的，尽管在当时竞争对手曾尝试过。经过300多年的显微镜改进，细胞的功能得到了令人着迷的发现。电子显微镜使用电子束而不是光，可以放大数十万倍。然而，被检查的材料虽然是及时修复的，但却是死的。光学显微镜随着激光技术、成像解决方案、计算机硬件/软件的发展以及荧光生物/化学探针的使用而发展。在过去的几十年里，最大的发展之一是共聚焦激光扫描显微镜，它允许用户在一个平面上生成固定材料的3D图像和简短的活细胞电影。随着共聚焦显微镜的发展，也出现了平行的发展/改进。其中一项发展是旋转圆盘技术，结合激光照明和高分辨率成像，允许用户以3D(4D)方式可视化细胞中的荧光探针，即在活细胞中。为了了解细胞中特定成分的作用和功能，这些特征对科学家来说是一个可取的优势。

项目成果

A thermodynamic model of microtubule assembly and disassembly.

• DOI: 10.1371/journal.pone.0006378
• 发表时间: 2009-08-11
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Piette BM;Liu J;Peeters K;Smertenko A;Hawkins T;Deeks M;Quinlan R;Zakrzewski WJ;Hussey PJ
• 通讯作者: Hussey PJ