Analysis of the signalling function of Arabidopsis cyclase associated protein (CAP1) and its interaction with a novel transmembrane protein (AtCIP).

分析拟南芥环化酶相关蛋白 (CAP1) 的信号功能及其与新型跨膜蛋白 (AtCIP) 的相互作用。

• 批准号: BB/E006256/1
• 负责人: P Hussey
• 金额: $ 35.74万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE006256%2F1
• 关键词: Analysis; signalling; function; Arabidopsis; cyclase

Each cell has an internal skeleton, the cytoskeleton. This is composed of three self-assembling protein filament systems: actin, microtubules and intermediate filaments. Plant cells appear only to have actin filaments and microtubules in their cytoskeleton. The cytoskeleton is responsible for many cellular processes including maintenance of cell structure, cell shape, mitosis (karyokinesis, the division of the chromosomes and cytokinesis, the division of the cell), the movement of organelles around the cell and cell expansion. The term 'Cytoskeleton' does not only include the filamentous elements but also a plethora of proteins that anchor, crossbridge or otherwise regulate the network within the cell. The cytoskeleton has to be responsive to cell signals that are generated internally or externally so that it can respond to the cells changing needs in different environments. For example, when a cell enters mitosis the cytoskeleton has to rearrange to divide the nuclei and the cell. The single celled amoeba has to move in the direction of food and the cytoskeleton governs this motility. Plants have to bend towards the light and to do this there has to be localised cell expansion. Actin and microtubules are structurally the same in animals, plants and fungae. Many of the proteins that bind and alter the dynamics of these filaments are also shared, but their sequence conservation is limited. The pathways used to regulate these proteins in plants are starting to be elucidated and significant differences are appearing. This is perhaps not surprising since the signals for cell growth and the environmental signals to which plants have to respond differ to those of animals. The focus of my laboratory is to determine the signalling pathways that control actin reorganisation in plants. The actin-binding Cyclase Associated Protein (CAP) has two roles; a signalling role and a role in controlling actin dynamics. The PJH laboratory has identified a mutation in the Arabidopsis CAP protein, and the appearance of the mutant is not what we would expect from just a defect in actin organisation. The question we are addressing here is 'What is the role of this protein in signalling in plants?'. Towards an answer to this question we have identified a plant specific protein that interacts with plant CAP and this protein localises to the plasma membrane offering the exciting possibility that the CAP1:AtCIP couple is part of a signal transduction machinery. We will be asking the question 'what is the function of the CAP1:AtCIP interaction?'

每个细胞都有一个内部骨架，细胞骨架。这是由三个自组装蛋白丝系统：肌动蛋白、微管和中间丝组成的。植物细胞在其细胞骨架中似乎只有肌动蛋白丝和微管。细胞骨架负责许多细胞过程，包括维持细胞结构，细胞形状，有丝分裂（核分裂，染色体分裂和细胞质分裂，细胞分裂），细胞周围细胞器的运动和细胞扩增。“细胞骨架”一词不仅包括丝状成分，还包括大量锚定、桥接或以其他方式调节细胞内网络的蛋白质。细胞骨架必须对内部或外部产生的细胞信号做出反应，这样它才能对不同环境中细胞变化的需求做出反应。例如，当细胞进入有丝分裂时，细胞骨架必须重新排列以分裂细胞核和细胞。单细胞变形虫必须向食物的方向移动，而细胞骨架控制着这种运动。植物必须向光弯曲，为了做到这一点，必须有局部的细胞扩张。动物、植物和真菌中的肌动蛋白和微管在结构上是相同的。许多结合和改变这些细丝动力学的蛋白质也是共享的，但它们的序列保守性是有限的。植物中用于调节这些蛋白质的途径开始被阐明，显著的差异正在出现。这也许并不奇怪，因为细胞生长的信号和植物必须响应的环境信号与动物的不同。我实验室的重点是确定控制植物中肌动蛋白重组的信号通路。肌动蛋白结合环化酶相关蛋白（CAP）有两个作用；信号作用和控制肌动蛋白动力学的作用。PJH实验室已经确定了拟南芥CAP蛋白的突变，并且突变的外观不是我们所期望的仅仅是肌动蛋白组织缺陷。我们在这里要解决的问题是“这种蛋白质在植物信号传递中的作用是什么？”为了回答这个问题，我们已经确定了一种与植物CAP相互作用的植物特异性蛋白质，这种蛋白质定位于质膜，提供了令人兴奋的可能性，即CAP1:AtCIP偶对是信号转导机制的一部分。我们会问“CAP1:AtCIP相互作用的功能是什么？”