Integrating genetics and high throughput genomics to identify genes underlying tomato QTL for metabolites that influence fruit quality (TOMQML)

整合遗传学和高通量基因组学来识别番茄 QTL 中影响果实品质的代谢物的基因 (TOMQML)

• 批准号: BB/G024901/1
• 负责人: Paul Fraser
• 金额: $ 38.15万
• 依托单位: Royal Holloway University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG024901%2F1
• 关键词: Integrating; genetics; throughput; genomics; identify

Fruits are an immensely important part of the human diet. Low fruit and vegetable intake is recognised as a major factor for increased risk of heart disease and certain cancers. Encouraging people to consume more fruit in their diet is not necessarily straight forward and depends on factors including cost and product quality. Some of the most important changes in fruit quality related to ripening involve the accumulation of primary metabolites such as sugars and acids which influence taste. Also alterations in the levels of secondary metabolites such as carotenoids and phenylpropanoids can have a significant impact on nutritional quality. The challenge is to understand how these individual ripening changes are controlled /modulated and develop commercially viable strategies for breeding novel and better fruit products. This project focuses on tomato as it is the most widely consumed fleshy fruit in the human diet, with a global value in excess of $10 bn. It has become the model for studying the development and ripening of fleshy fruits and a wealth of genetic and genomic resources are available. We will use a range of well characterised publicly available tomato lines and novel tomato genetic resources combined with a range of genetic and molecular approaches to identify the genes controlling tomato taste and the accumulation of health enhancing phytochemicals. In concert with these approaches, we will identify the links between major regulators of ripening and their associated metabolite changes. The project will make an important contribution to fundamental knowledge about the molecular basis of fruit ripening. The consortium consists of internationally recognized groups from universities and research institutes in Europe and the USA as well as a global supplier of vegetable seeds, collectively ensuring a framework for innovative excellence.

水果是人类饮食中非常重要的一部分。水果和蔬菜摄入量低被认为是心脏病和某些癌症风险增加的主要因素。鼓励人们在饮食中多吃水果并不一定是直截了当的，这取决于成本和产品质量等因素。与成熟有关的水果品质的一些最重要的变化涉及影响味道的初级代谢产物如糖和酸的积累。次级代谢物如类胡萝卜素和苯丙素水平的改变也会对营养质量产生重大影响。挑战在于了解这些个体成熟变化是如何被控制/调节的，并开发商业上可行的策略来培育新的和更好的水果产品。该项目的重点是番茄，因为它是人类饮食中消费最广泛的肉质水果，全球价值超过100亿美元。它已成为研究肉质果实发育和成熟的模式，并提供了丰富的遗传和基因组资源。我们将使用一系列具有良好特征的公开可用的番茄品系和新型番茄遗传资源，结合一系列遗传和分子方法，以确定控制番茄味道的基因和增强健康的植物化学物质的积累。与这些方法相一致，我们将确定成熟的主要调节剂及其相关的代谢物变化之间的联系。该项目将对水果成熟的分子基础的基础知识作出重要贡献。该联盟由来自欧洲和美国的大学和研究机构的国际知名团体以及全球蔬菜种子供应商组成，共同确保创新卓越的框架。

项目成果

A genome-wide metabolomic resource for tomato fruit from Solanum pennellii.

• DOI: 10.1038/srep03859
• 发表时间: 2014-01-24
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Perez-Fons L;Wells T;Corol DI;Ward JL;Gerrish C;Beale MH;Seymour GB;Bramley PM;Fraser PD
• 通讯作者: Fraser PD

Metabolite profiling in LC-DAD using multivariate curve resolution: the alsace package for R

使用多元曲线分辨率在 LC-DAD 中进行代谢物分析：R 的 alsace 包

• DOI: 10.1007/s11306-014-0683-5
• 发表时间: 2014
• 期刊: Metabolomics
• 影响因子: 3.6
• 作者: Wehrens R

The identification and rapid extraction of hydrocarbons from Nicotiana glauca: A potential advanced renewable biofuel source

从青烟草中鉴定并快速提取碳氢化合物：一种潜在的先进可再生生物燃料来源

• DOI: 10.1016/j.phytol.2012.04.004
• 发表时间: 2012
• 期刊: Phytochemistry Letters
• 影响因子: 1.7
• 作者: Mortimer C