A novel plant pathogenesis regulatory system in Erwinia: functional analysis of a new post-transcriptional input to bacterial quorum sensing control.

欧文氏菌的一种新型植物发病机制调节系统：细菌群体感应控制的新转录后输入的功能分析。

• 批准号: BB/H013261/1
• 负责人: George Salmond
• 金额: $ 49.53万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FH013261%2F1
• 关键词: novel; plant; pathogenesis; regulatory; system

This proposed research involves the study of a new gene in a bacterial pathogen of plants. The bacterial pathogen (Erwinia) causes rotting diseases of potato and other plants and is important in potato crop production. The bacteria attack the plant by producing a spectrum of enzymes that can degrade the cell walls of the plant leading to the commercially-significant rotting disease symptoms. The ability of the bacteria to make these plant cell wall degrading enzymes is affected by the cell population density of the bacteria; the enzymes are only made in abundance when the bacterial density if high. The bacteria make a small diffusible chemical signal called OHHL and the concentration of this molecule is a direct reflection of bacterial density. In this process (quorum sensing) the bacteria use the chemical signal to communicate with each other and thereby link cell density to the ability to aggressively rot the plant. If the ability to enact the process of quorum sensing is blocked then the bacteria are no longer capable of causing disease. Consequently, a deeper understanding of the mode of action of quorum sensing in this potato pathogen is important for our fundamental appreciation of how bacterial pathogens communicate with each other and, if we can understand the process, how we might intervene in the disease. There are no chemical control systems available for potato diseases caused by Erwinia and so fundamental understanding of how it causes infection is the only route to an eventual rational therapy. In this proposal we will investigate the role of a new gene (ECA2020) that we have shown recently to be involved in the process of quorum sensing. The product of this gene affects the expression/functionality of one of the key proteins (VirR) involved in quorum sensing. We will study the way ECA2020 operates by investigating which other proteins it interacts with and we will test the hypothesis that it may have some functions similar to proteins from higher organisms that are involved in messenger RNA processing. We will look for bacterial mutants that can bypass their dependence on the ECA2020 system and we will study the precise impact on production of the fully active VirR protein. We will also test the possibility that the contiguous gene (ECA2019) might be involved in a related process and we will investigate how the ECA2020 gene is itself regulated. The overall aim is to try to work out how this new gene modulates the function of the quorum sensing system during plant infection and disease initiation because this might be a target in the longer term for control of potato rotting diseases caused by Erwinia.

这项拟议中的研究涉及对植物细菌病原体中一种新基因的研究。细菌性病原菌（欧文氏菌属）引起马铃薯和其他植物的腐烂病，在马铃薯作物生产中很重要。细菌通过产生一系列酶来攻击植物，这些酶可以降解植物的细胞壁，导致商业上显著的腐烂疾病症状。细菌产生这些植物细胞壁降解酶的能力受细菌的细胞群体密度的影响;只有当细菌密度高时，才能大量产生酶。细菌会产生一种称为OHHL的小的可扩散化学信号，该分子的浓度直接反映了细菌密度。在这个过程中（群体感应），细菌使用化学信号相互交流，从而将细胞密度与积极腐烂植物的能力联系起来。如果制定群体感应过程的能力被阻断，那么细菌就不再能够引起疾病。因此，更深入地了解这种马铃薯病原体中群体感应的作用模式对于我们从根本上理解细菌病原体如何相互交流以及如果我们能够理解这一过程，我们如何干预疾病非常重要。没有化学控制系统可用于由欧文氏菌引起的马铃薯疾病，因此从根本上了解它如何引起感染是最终合理治疗的唯一途径。在这个提议中，我们将研究一个新的基因（ECA 2020）的作用，我们最近已经表明，参与群体感应的过程。该基因的产物影响参与群体感应的关键蛋白之一（VirR）的表达/功能。我们将通过调查它与哪些其他蛋白质相互作用来研究ECA 2020的运作方式，我们将测试它可能具有与参与信使RNA加工的高等生物蛋白质相似的功能的假设。我们将寻找可以绕过对ECA 2020系统依赖的细菌突变体，并研究对完全活性VirR蛋白生产的精确影响。我们还将测试连续基因（ECA 2019）可能参与相关过程的可能性，并研究ECA 2020基因本身是如何调节的。总体目标是试图弄清楚这个新基因如何在植物感染和疾病引发期间调节群体感应系统的功能，因为这可能是控制欧文氏菌引起的马铃薯腐烂疾病的长期目标。

项目成果

Intra-species bacterial quorum sensing studied at single cell level in a double droplet trapping system.

在双液滴诱捕系统中，在单细胞水平上研究了种类的细菌群体传感。

• DOI: 10.3390/ijms140510570
• 发表时间: 2013-05-21
• 期刊: International journal of molecular sciences
• 影响因子: 5.6
• 作者: Bai Y;Patil SN;Bowden SD;Poulter S;Pan J;Salmond GP;Welch M;Huck WT;Abell C
• 通讯作者: Abell C

The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens.

rsmS (ybaM) 突变导致肠细菌植物病原体中 RsmAB 转录后毒力调节系统的旁路抑制。

• DOI: 10.1038/s41598-019-40970-3
• 发表时间: 2019
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Monson RE

A Plasmid-Transposon Hybrid Mutagenesis System Effective in a Broad Range of Enterobacteria.

• DOI: 10.3389/fmicb.2015.01442
• 发表时间: 2015
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Monson R;Smith DS;Matilla MA;Roberts K;Richardson E;Drew A;Williamson N;Ramsay J;Welch M;Salmond GP
• 通讯作者: Salmond GP

Robust routes for the synthesis of N-acylated-L-homoserine lactone (AHL) quorum sensing molecules with high levels of enantiomeric purity

• DOI: 10.1016/j.tetlet.2011.04.059
• 发表时间: 2011-06-29
• 期刊: TETRAHEDRON LETTERS
• 影响因子: 1.8
• 作者: Hodgkinson, James T.;Galloway, Warren R. J. D.;Spring, David R.
• 通讯作者: Spring, David R.

Draft Genome Sequence of Serratia sp. Strain ATCC 39006, a Model Bacterium for Analysis of the Biosynthesis and Regulation of Prodigiosin, a Carbapenem, and Gas Vesicles.

• DOI: 10.1128/genomea.01039-13
• 发表时间: 2013-12-12
• 期刊: Genome announcements
• 作者: Fineran PC;Iglesias Cans MC;Ramsay JP;Wilf NM;Cossyleon D;McNeil MB;Williamson NR;Monson RE;Becher SA;Stanton JA;Brügger K;Brown SD;Salmond GP
• 通讯作者: Salmond GP